Phospho-Cofilin (Ser3) (77G2) Rabbit mAb

• 产品编号：CST-3313L      品牌：CST       原厂货号：3313L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb仅识别serine 3磷酸化的内源性cofilin蛋白。通过人工合成人源cofilin蛋白Ser3邻近氨基酸序列一致的肽段，免疫动物从而制备出单克隆抗体。Cofilin和actin-解聚因子（ADF）都属于一组保守的小actin-结合蛋白家族，在胞质分裂，内吞，胚胎发育，压力应激和组织再生过程中发挥了关键作用（1）。在刺激因素作用下，cofilin作为提前存在的细丝促进actin肌动蛋白丝的再生（2）。Cofilin的第3位丝氨酸被LIMK或TESK磷酸化后会抑制cofilin的这种作用（3-5）。第3位丝氨酸磷酸化也会调控cofilin从细胞核转位到细胞质中（6）。

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

原厂资料：

Specificity / Sensitivity

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb detects endogenous levels of cofilin only when phosphorylated at serine 3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin.

Background

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody