Phospho-Cofilin (Ser3) Antibody

• 产品编号：CST-3311L      品牌：CST       原厂货号：3311L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Cofilin (Ser3) 抗体仅识别serine 3磷酸化的内源性cofilin总蛋白。该抗体可能与磷酸化的cofilin 2（muscle isoform）发生交叉反应。通过合成与人源cofilin蛋白Ser3邻近氨基酸残基序列一致的肽段，免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Cofilin和actin-解聚因子（ADF）都属于一组保守的小actin-结合蛋白家族，在胞质分裂，内吞，胚胎发育，压力应激和组织再生过程中发挥了关键作用（1）。在刺激因素作用下，cofilin作为提前存在的细丝促进actin肌动蛋白丝的再生（2）。Cofilin的第3位丝氨酸被LIMK或TESK磷酸化后会抑制cofilin的这种作用（3-5）。第3位丝氨酸磷酸化也会调控cofilin从细胞核转位到细胞质中（6）。

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

Phospho-Cofilin (Ser3) Antibody detects endogenous levels of cofilin only when phosphorylated at serine 3. The antibody may cross-react with phosphorylated cofilin 2, the muscle isoform.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
3. Arber, S. et al. (1998) Nature 393, 805-9.
4. Yang, N. et al. (1998) Nature 393, 809-12.
5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.