GCN5L2 (C26A10) Rabbit mAb

• 产品编号：CST-3305S      品牌：CST       原厂货号：3305S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GCN5L2 (C26A10) Rabbit mAb兔单抗能够检测内源性GCN5L2总蛋白水平。该抗体不能与相关的PCAF蛋白发生交叉反应。通过合成的与人源GCN5L2蛋白氨基端相应的多肽片段去免疫动物从而制备出此单克隆抗体。General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2)是一个转录接头蛋白和一个组蛋白乙酰转移酶(HAT)，它的功能作为STAGA和TFTC转录共激活因子复合物的催化亚单位(1)。GCN5L2蛋白与p300/CBP-associated factor PCAF有73%同源性，另外HAT 蛋白也发现在相同的复合物中(2)。游离的GCN5L2蛋白使histone H3在Lys14位点发生乙酰化；然而，当它是共激活复合物中的一部分时，GCN5L2蛋白使histone H3在Lys9、14、18和23 ，以及更小程度的histones H4 and H2B发生乙酰化(3)。组蛋白乙酰化有助于基因激活，这主要通过调节染色质结构和招募附加的共激活因子蛋白，这些蛋白包含有acetyl-lysine binding bromodomains(4)。GCN5L2也使非组蛋白发生乙酰化例如转录激活因子(TAT, c-Myb) (5,6)、转录共激活因子 (PGC1-α) (7)和细胞核受体(Steroidogenic Factor 1) (8)。这些蛋白的乙酰化调节它们的细胞核定位、蛋白质稳定、DNA结合和共激活因子联系(5-8)。在超激活期间通过与多种转录激活蛋白例如Myc、E2F、p53和BRCA1蛋白相互作用，GCN5L2 蛋白被招募到基因的启动子区域(9-12)。STAGA和TFTC复合物也与涉及RNA剪切和DNA修复的两个结构性相关的蛋白质SAP130和DDB1发生相互作用，这认为GCN5L2在加工过程作用不同于转录激活(13)。

1. Candau, R. et al. (1996) Mol Cell Biol 16, 593-602.
2. Yang, X.J. et al. (1996) Nature 382, 319-24.
3. Grant, P.A. et al. (1999) J Biol Chem 274, 5895-900.
4. Yang, X.J. (2004) Bioessays 26, 1076-87.
5. Kiernan, R.E. et al. (1999) EMBO J 18, 6106-18.
6. Tomita, A. et al. (2000) Oncogene 19, 444-51.
7. Lerin, C. et al. (2006) Cell Metab 3, 429-38.
8. Jacob, A.L. et al. (2001) J Biol Chem 276, 37659-64.
9. Liu, X. et al. (2003) J Biol Chem 278, 20405-12.
10. Lang, S.E. et al. (2001) J Biol Chem 276, 32627-34.
11. Candau, R. et al. (1997) Oncogene 15, 807-16.
12. Oishi, H. et al. (2006) J Biol Chem 281, 20-6.
13. Brand, M. et al. (2001) EMBO J 20, 3187-96.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Horse

Specificity / Sensitivity

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human GCN5L2.

Background

General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) is a transcription adaptor protein and a histone acetyltransferase (HAT) that functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (1). GCN5L2 is 73% homologous to the p300/CBP-associated factor PCAF, another HAT protein found in similar complexes (2). Free GCN5L2 acetylates histone H3 on Lys14; however, when part of coactivator complexes, GCN5L2 acetylates histone H3 at Lys9, 14, 18, and 23, and to a smaller extent histones H4 and H2B (3). Histone acetylation contributes to gene activation by modulating chromatin structure and recruiting additional coactivator proteins that contain acetyl-lysine binding bromodomains (4). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (5,6), transcription co-activators (PGC1-α) (7), and nuclear receptors (Steroidogenic Factor 1) (8). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (5-8). GCN5L2 is recruited to gene promoters during transactivation through interactions with multiple transcription activator proteins such as Myc, E2F, p53, and BRCA1 (9-12). The STAGA and TFTC complexes also interact with SAP130 and DDB1, two structurally related proteins involved in RNA splicing and DNA repair, suggesting roles for GCN5L2 in processes other than transcription activation (13).

1. Candau, R. et al. (1996) Mol Cell Biol 16, 593-602.
2. Yang, X.J. et al. (1996) Nature 382, 319-24.
3. Grant, P.A. et al. (1999) J Biol Chem 274, 5895-900.
4. Yang, X.J. (2004) Bioessays 26, 1076-87.
5. Kiernan, R.E. et al. (1999) EMBO J 18, 6106-18.
6. Tomita, A. et al. (2000) Oncogene 19, 444-51.
7. Lerin, C. et al. (2006) Cell Metab 3, 429-38.
8. Jacob, A.L. et al. (2001) J Biol Chem 276, 37659-64.
9. Liu, X. et al. (2003) J Biol Chem 278, 20405-12.
10. Lang, S.E. et al. (2001) J Biol Chem 276, 32627-34.
11. Candau, R. et al. (1997) Oncogene 15, 807-16.
12. Oishi, H. et al. (2006) J Biol Chem 281, 20-6.
13. Brand, M. et al. (2001) EMBO J 20, 3187-96.

GCN5L2 (C26A10) Rabbit mAb detects endogenous levels of total GCN5L2 protein. The antibody does not cross-react with the related PCAF protein.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.