EEA1 (C45B10) Rabbit mAb

• 产品编号：CST-3288S      品牌：CST       原厂货号：3288S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EEA1 (C45B10) Rabbit mAb识别内源性的EEA1总蛋白。该单克隆抗体用与人类EEA1蛋白中Ser70位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。EEA1是一个早期内涵体标志物，也是Rab5效应蛋白，对内涵体膜融合和转运至关重要（1-2）。EEA1羧基末端包括一个FYVE结构域，可以结合到磷脂酰肌醇-3-磷酸酯（PtdIns(3)P），将EEA1定位到早期内体（3）。EEA1与内体膜的稳定结合由PI3激酶、Rab5和钙/钙调蛋白（4-6）。一旦到膜上，EEA1就能与Rab5、NSF和syntaxin 13相互作用，促进早期内体膜的锚定与融合（7）。

1. Mu, F.T. et al. (1995) J. Biol. Chem. 270, 13503-13511.
2. Christoforidis, S. et al. (1999) Nature 397, 621-625.
3. Gaullier, J.M. et al. (1998) Nature 394, 432-433.
4. Patki, V. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 7326-7330.
5. Lawe, D.C. et al. (2003) Mol. Biol. Cell 14, 2935-2945.
6. Simonsen, A. et al. (1998) Nature 394, 494-498.
7. McBride, H.M. et al. (1999) Cell 98, 377-386.

原厂资料：

Specificity / Sensitivity

EEA1 (C45B10) Rabbit mAb detects endogenous levels of total EEA1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein.

Background

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).

1. Mu, F.T. et al. (1995) J. Biol. Chem. 270, 13503-13511.
2. Christoforidis, S. et al. (1999) Nature 397, 621-625.
3. Gaullier, J.M. et al. (1998) Nature 394, 432-433.
4. Patki, V. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 7326-7330.
5. Lawe, D.C. et al. (2003) Mol. Biol. Cell 14, 2935-2945.
6. Simonsen, A. et al. (1998) Nature 394, 494-498.
7. McBride, H.M. et al. (1999) Cell 98, 377-386.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.