Axl (L283) Antibody

• 产品编号：CST-3269S      品牌：CST       原厂货号：3269S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Axl (L283)抗体能检测内源总Axl 蛋白水平。本抗体不与其它相关蛋白交叉反应。多克隆抗体通过免疫动物得到，该合成多肽是根据人的Axl蛋白胞外区Leu283附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。Axl、 Sky和 Mer是 受体酪氨酸激酶 (RTK) 家族的三个成员，它们有共同的胞内酪氨酸激酶区域和与胞外粘附分子相似的胞外区。这些受体酪氨酸激酶与维生素K依赖性的生长停滞特异性6蛋白（Gas6）结合，Gas6蛋白与S抗凝血因子蛋白结构上相关(1)。与受体结合后，Gas6能够激活PI3K激酶和它的下游靶分子Akt、 S6K和 NF-κB (2,3)。大量证据表明Gas6/Axl信号通路与正常细胞和癌细胞的生长和存活相关(4)。

1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.

原厂资料：

Specificity / Sensitivity

Axl (L283) Antibody detects endogenous levels of total Axl protein. The antibody does not cross-react with related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequences surrounding amino acid residue Leu283 of the human Axl extracellular domain. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

Axl, Sky and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-135.
2. Demarchi, F. et al. (2001) J. Biol. Chem. 276, 31738-31744.
3. Lee, W. P. et al. (2002) Oncogene 21, 329-336.
4. Bellosta, P. et al. (1997) Oncogene 15, 2387-2397.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.