Blk Antibody

• 产品编号：CST-3262S      品牌：CST       原厂货号：3262S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Blk抗体能够检测内源Blk 蛋白总体水平。此多克隆抗体是通过合成人源对应的Blk C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化Blk是Src家族蛋白酪氨酸激酶，在B细胞发育过程中的每个阶段都有表达(1,2)。通过各种配体激活的B细胞都伴随着Blk的激活(3)。研究表明Blk参与到B细胞分化和增值的调控过程中(4,5)。Blk转录在人的胸腺细胞中检测到，但是在成熟的T细胞中检测不到，这就暗示Blk在功能的胸腺产生的T淋巴细胞中发挥了重要的作用(6)。Blk的功能可能是冗余的，然而，当小鼠不表达Blk对于B细胞的发育和免疫应答并没有缺陷(7)。

1. Dymecki, S.M. et al. (1990) Science 247, 332-336.
2. Wasserman, R. et al. (1995) J. Immunol. 155, 644-651.
3. Sefton, B.M. et al. (1994) Curr. Opin. Immunol. 6, 372-379.
4. Malek, S.N. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 7351-7356.
5. Yao, X.R. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 7946-7950.
6. Islam, K.B. et al. (1995) J. Immunol. 154, 1265-1272.
7. Texido, G. et al. (2000) Mol. Cell Biol. 20, 1227-1233.

原厂资料：

Specificity / Sensitivity

Blk Antibody detects endogenous levels of total Blk.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy-terminus of human Blk. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of Blk (3). It has been suggested that Blk is involved in the control of B cell differentiation and proliferation (4,5). Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis (6). Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response (7).

1. Dymecki, S.M. et al. (1990) Science 247, 332-336.
2. Wasserman, R. et al. (1995) J. Immunol. 155, 644-651.
3. Sefton, B.M. et al. (1994) Curr. Opin. Immunol. 6, 372-379.
4. Malek, S.N. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 7351-7356.
5. Yao, X.R. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 7946-7950.
6. Islam, K.B. et al. (1995) J. Immunol. 154, 1265-1272.
7. Texido, G. et al. (2000) Mol. Cell Biol. 20, 1227-1233.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.