DR3 Antibody

• 产品编号：CST-3254S      品牌：Cell Signaling Technology       原厂货号：3254S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DR3 Antibody 能够检测内源的人源DR3蛋白。该抗体不会与TNF受体家族产生交叉反应。该DR3 Antibody是采用合成的人源DR3蛋白细胞内结构域周围残基相对应的肽段免疫兔子而制备的。抗体由protein A和肽亲和层析技术纯化。肿瘤坏死因子受体家族，包括TNF-RI、FAS、DR3、DR4、DR5和DR6，在各个生理系统中，在细胞凋亡的调控中起着重要的作用（1,2）。受体被一个家族的细胞因子激活，包括TNF、FasL和TRAIL。它们的特点是含有一个高度保守、富含半胱氨酸的重复序列的胞外区和一个胞内保守的约80个氨基酸的死亡结构域（DD）。 DD对于死亡信号转导的传递很重要，它通过招募其他的DD配体蛋白（FADD，TRADD，RIP）到死亡诱导信号复合物（DISC）来发挥作用，进而激活半胱天冬酶蛋白。DR3/WSL-1/Apo-3/TRAMP/LARD是一种肿瘤坏死因子 TNFR 受体家族成员，包含特征性的细胞外的外半胱氨酸重复，跨膜区和细胞内的DD（3-7）。 DR3被其配体Apo-3L/TWEAK激活，进而诱导细胞凋亡和NF-κB的活化（8,9）。像TNF-R1一样，DR3 结合DD接头蛋白TRADD，然后可以与其他DD蛋白质，如FADD，RIP和TRAF家族的成员相互作用（3,4）。 DR3的组织表达非常严格，主要在激活的胸腺细胞和淋巴细胞的表面上看到，这在胸腺细胞的负选择中起着重要的作用（3,4,10）。研究还表明，DR3和类风湿关节炎间存在关系（11,12）。

1. Nagata, S. (1997) Cell 88, 355-365.
2. Thorburn, A. (2004) Cell. Signal. 16, 139-144.
3. Chinnaiyan, A.M. et al. (1996) Science 274, 990-992.
4. Kitson, J. et al. (1996) Nature 384, 372-375.
5. Marsters, S.A. et al. (1996) Curr. Biol. 6, 1669-1676.
6. Bodmer, J.L. et al. (1997) Immunity 6, 79-88.
7. Screaton, G.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4615-4619.
8. Marsters, S.A. et al. (1998) Curr. Biol. 8, 525-528.
9. Kaptein, A. et al. (2000) FEBS Lett. 485, 135-141.
10. Wang, E.C. et al. (2001) Mol. Cell Biol. 21, 3451-3461.
11. Osawa, K. et al. (2004) Genes Immun. 5, 439-443.
12. Borysenko, C.W. et al. (2005) Biochem. Biophys. Res. Commun. 328, 794-799.

原厂资料：

Specificity / Sensitivity

DR3 Antibody detects endogenous levels of human DR3. It is not expected to cross-react with other TNF receptor family members.

Source / Purification

DR3 Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to residues within the intracellular domain of human DR3. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.DR3/WSL-1/Apo-3/TRAMP/LARD is a TNFR family member containing the characteristic extracellular cysteine-repeats, transmembrane region, and intracellular DD (3-7). DR3 is activated by its ligand Apo-3L/TWEAK to induce apoptosis and activation of NF-κB (8,9). Like TNF-R1, DR3 binds to the DD adaptor protein TRADD, which can then associate with other DD proteins like FADD and RIP as well as members of the TRAF family (3,4). Tissue expression of DR3 is very restricted, primarily seen on the surface of activated thymocytes and lymphocytes and plays an important role in thymocyte negative selection (3,4,10). Studies have also indicated an association with DR3 and rheumatoid arthritis (11,12).

1. Nagata, S. (1997) Cell 88, 355-365.
2. Thorburn, A. (2004) Cell. Signal. 16, 139-144.
3. Chinnaiyan, A.M. et al. (1996) Science 274, 990-992.
4. Kitson, J. et al. (1996) Nature 384, 372-375.
5. Marsters, S.A. et al. (1996) Curr. Biol. 6, 1669-1676.
6. Bodmer, J.L. et al. (1997) Immunity 6, 79-88.
7. Screaton, G.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4615-4619.
8. Marsters, S.A. et al. (1998) Curr. Biol. 8, 525-528.
9. Kaptein, A. et al. (2000) FEBS Lett. 485, 135-141.
10. Wang, E.C. et al. (2001) Mol. Cell Biol. 21, 3451-3461.
11. Osawa, K. et al. (2004) Genes Immun. 5, 439-443.
12. Borysenko, C.W. et al. (2005) Biochem. Biophys. Res. Commun. 328, 794-799.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.