α-E-Catenin Antibody

• 产品编号：CST-3236S      品牌：CST       原厂货号：3236S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

α-E-Catenin抗体检测内源性alpha-E catenin蛋白的水平，抗体可能与神经alpha-N- catenin交叉反应，在一些细胞裂解物种，抗体可能与一个50kDa的未知条带交叉反应。多克隆抗体由合成的 肽段免疫动物产生，该肽段与人α-E-Catenin的羧基末端序列对应。抗体由蛋白A和肽段亲和层析纯化得到。粘着连接是一种形成细胞间接触的动力结构， 在发育、分化、组织完整性、形态学和细胞极性中很重要。它们由跨膜蛋白cadherins组成，cadherin以钙依赖的方式在临近细胞上结合cadherin。在粘着连接细胞质的一边，经典模型说明cadherins通过β- 和 α-catenin与细胞骨架联系。α-E-catenin广泛表达，α-N-catenin在神经组织中表达，α-T-catenin主要在心脏组织中表达。E-cadherin 和α-E-catenin的减少发生在一些人类癌症进程中，说明粘着连接的降解对于癌症进程的重要性（综述1）。近来证据显示，在cadherins 和 actin之间不是静态连接，α-catenin直接调控肌动蛋白动力学机制，很可能通过和肌动蛋白竞争成核arp2/3复合物（2,3）. α-catenin也起作用调控β-catenin依赖的转录活性，影响分化和对Wnt信号通路的反应。α-catenin在核内结合β-catenin，阻止它调控转录，两种蛋白的水平被蛋白酶体依赖的降解所调控（4）。

1. Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
2. Yamada, S. et al. (2005) Cell 123, 889-901.
3. Drees, F. et al. (2005) Cell 123, 903-15.
4. Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.

原厂资料：

Specificity / Sensitivity

α-E-Catenin Antibody detects endogenous levels of alpha-E catenin protein. The antibody may cross-react with neuronal α-N-catenin. In some cell lysates, the antibody may cross-react with a 50 kDa band of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human α-E-Catenin. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). 
 Recent evidence suggests that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).

1. Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25.
2. Yamada, S. et al. (2005) Cell 123, 889-901.
3. Drees, F. et al. (2005) Cell 123, 903-15.
4. Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody