Phospho-Gab1 (Tyr307) Antibody

• 产品编号：CST-3234P      品牌：Cell Signaling Technology       原厂货号：3234P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Gab1 (Tyr307) Antibody 能够检测酪氨酸（307位点）磷酸化的内源性Gab1蛋白。抗体与磷酸化的Gab2及可能还有磷酸化的Gab3交叉反应。抗体也可能于活化的受体酪氨酸激酶(RTKs)交叉反应。该多克隆抗体是由合成与人源Gab1蛋白酪氨酸（307位点）周围序列相对应的磷酸化肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。Grb相关结合因子（Gab）家族是一类由多种受体酪氨酸激酶（RTKs），如EGFR，HGFR，胰岛素受体，细胞因子受体和B细胞抗原受体招募的接头蛋白。RTKs受到它们的同源配体刺激后，Gab被招募到细胞膜上，接着其被磷酸化，并起到支架的作用（1-4）。Gab1蛋白存在多个酪氨酸磷酸化位点（5）。酪氨酸（472位点）磷酸化可以调节其与PI3K激酶p85的结合（6,7）。Gab1酪氨酸（307，373和407位点）的磷酸化可以调节Gab1与PLCγ的互作（8）。酪氨酸（627和659位点）磷酸化是Gab1结合并活化蛋白酪氨酸磷酸酶SHP2所必需的（6,9）。

1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.

原厂资料：

Specificity / Sensitivity

Phospho-Gab1 (Tyr307) Antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 307. The antibody cross-reacts with phosphorylated Gab2 and may also cross-react with phosphorylated Gab3. The antibody may cross-react with activated receptor tyrosine kinases (RTKs).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr307 of human Gab1. Antibodies are purified by protein A and peptide affinity chromatography.

ackground

The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).

1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody