GβL Antibody

• 产品编号：CST-3227S      品牌：CST       原厂货号：3227S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GβL Antibody 能够检测内源性的GβL总蛋白。该多克隆抗体是采用人类GβL蛋白Gln210周围残基相对应的合成肽段免疫动物生产的。抗体由protein A和肽亲和层析技术纯化。细胞生长是一个细胞增加物质扩大体积的基本生物学过程。哺乳动物雷帕霉素靶点（mTOR）通路和生长因子驱动的信号一起协调能量和营养信号，调节生长（1）。mTOR是一个大的具有两种形式复合物的蛋白激酶。一个复合物包含mTOR，GβL和raptor，是雷帕霉素的靶点。另一个复合物，对雷帕霉素不敏感，包括mTOR，GβL，和rictor（1）。GβL与mTOR激酶结构域结合并激活mTOR激酶活性（2）。人们已证明GβL表达减少可以减少S6K1体内磷酸化水平（2）。

1. Sarbassov, D.D. et al. (2004) Curr Biol. 14, 1296-1302.
2. Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.

原厂资料：

Specificity / Sensitivity

GβL Antibody detects endogenous levels of total GβL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln164 of human GβL. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).

1. Sarbassov, D.D. et al. (2004) Curr Biol. 14, 1296-1302.
2. Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.