Dvl2 (30D2) Rabbit mAb

• 产品编号：CST-3224P      品牌：Cell Signaling Technology       原厂货号：3224P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Dvl2 (30D2)Rabbit mAb兔单抗识别内源性的总Dvl2 蛋白.单抗由合成肽段免疫动物产生，该合成肽段与邻近人源Dvl2蛋白的Gly176氨基酸残基序列一致.Dishevelled (Dsh)蛋白是重要的Wnt信号通路中间调控因子。Dsh抑制糖原合成酶激酶3β，促进β-catenin蛋白稳定，Dsh蛋白通过激活JNK参与平面细胞极化通路（1,2）。有三种哺乳动物Dsh同源体Dvl1, Dvl2和Dvl3，在Wnt蛋白加入后，Dvls被高度磷酸化（3）并积累在核内（4）。Dvl蛋白也和肌动蛋白纤维和细胞质液泡膜相关（5）。并在Wnt蛋白刺激后调控Fzd受体细胞吞噬（6）。也有Dvl在癌症中过表达的报道（7,8）.

1. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
2. Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37.
3. Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70.
4. Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12.
5. Capelluto, D.G. et al. (2002) Nature 419, 726-729.
6. Chen, W. et al. (2003) Science 301, 1391-1394.
7. Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223.
8. Uematsu, K. et al. (2003) Oncogene 22, 7218-7221.

原厂资料：

Specificity / Sensitivity

Dvl2 (30D2) Rabbit mAb detects endogenous levels of total Dvl2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly176 of human Dvl2.

Background

Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers 

1. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
2. Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37.
3. Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70.
4. Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12.
5. Capelluto, D.G. et al. (2002) Nature 419, 726-729.
6. Chen, W. et al. (2003) Science 301, 1391-1394.
7. Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223.
8. Uematsu, K. et al. (2003) Oncogene 22, 7218-7221.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.