Phospho-Ret (Tyr905) Antibody

• 产品编号：CST-3221S      品牌：CST       原厂货号：3221S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化Ret (Tyr905) 抗体仅在Ret蛋白的tyrosine 905位点磷酸化时能检测到内源的Ret蛋白的表达。多克隆抗体通过用合成的磷酸化多肽免疫动物得到，该多肽是根据人的Ret蛋白Tyr905附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。Ret原癌基因（c-Ret）是一个多功能的酪氨酸激酶受体复合物，它和其它膜结合的GDNF家族的配体结合型受体共同发挥作用（1）。与Ret受体结合的配体包括神经胶质细胞来源的神经营养因子（GDNF）和它的同类物neurturin、 persephin 和 artemin (2-4)。在相应的Ret 基因的改变会引起乳突状甲状腺癌、多发性内分泌肿瘤（2A和2B型），家族性髓样甲状腺癌和先天性巨结肠病(1,3)。位于Ret激酶区的Tyr905位点对于Ret的催化功能和生物学功能十分重要。替换Tyr905的Phe能显著抑制Ret的自磷酸化活性（5）。

1. Airaksinen, M.S. et al. (1999) Mol Cell Neurosci 13, 313-25.
2. Takahashi, M. et al. (1989) Oncogene 4, 805-6.
3. Manié, S. et al. (2001) Trends Genet 17, 580-9.
4. Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
5. Iwashita, T. et al. (1999) Oncogene 18, 3919-22.
6. Plaza-Menacho, I. et al. (2014) Mol Cell 53, 738-51.

原厂资料：

Specificity / Sensitivity

Phospho-Ret (Tyr905) Antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 905.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4). Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity (5).

1. Airaksinen, M.S. et al. (1999) Mol Cell Neurosci 13, 313-25.
2. Takahashi, M. et al. (1989) Oncogene 4, 805-6.
3. Manié, S. et al. (2001) Trends Genet 17, 580-9.
4. Tallini, G. and Asa, S.L. (2001) Adv Anat Pathol 8, 345-54.
5. Iwashita, T. et al. (1999) Oncogene 18, 3919-22.
6. Plaza-Menacho, I. et al. (2014) Mol Cell 53, 738-51.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.