Dvl3 Antibody

• 产品编号：CST-3218S      品牌：CST       原厂货号：3218S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Dvl3抗体识别内源性的总Dvl3蛋白，这种抗体不与Dvl2交叉反应.

多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Dvl3蛋白的C末端氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化.Dishevelled (Dsh)蛋白是重要的Wnt信号通路中间调控因子。Dsh抑制糖原合成酶激酶3β，促进β-catenin蛋白稳定，Dsh蛋白通过激活JNK参与平面细胞极化通路（1,2）。有三种哺乳动物Dsh同源体Dvl1, Dvl2和Dvl3，在Wnt蛋白加入后，Dvls被高度磷酸化（3）并积累在核内（4）。Dvl蛋白也和肌动蛋白纤维和细胞质液泡膜相关（5）。并在Wnt蛋白刺激后调控Fzd受体细胞吞噬（6）。也有Dvl在癌症中过表达的报道（7,8）.

1. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
2. Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37.
3. Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70.
4. Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12.
5. Capelluto, D.G. et al. (2002) Nature 419, 726-729.
6. Chen, W. et al. (2003) Science 301, 1391-1394.
7. Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223.
8. Uematsu, K. et al. (2003) Oncogene 22, 7218-7221.

原厂资料：

Specificity / Sensitivity

Dvl3 Antibody detects endogenous levels of total Dvl3 protein. This antibody does not cross-react with Dvl2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human Dvl3. The antibodies are purified by protein A and peptide affinity chromatography.

Background

Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8).

1. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
2. Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37.
3. Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70.
4. Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12.
5. Capelluto, D.G. et al. (2002) Nature 419, 726-729.
6. Chen, W. et al. (2003) Science 301, 1391-1394.
7. Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223.
8. Uematsu, K. et al. (2003) Oncogene 22, 7218-7221.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.