Phospho-Etk (Tyr40) Antibody

• 产品编号：CST-3211S      品牌：CST       原厂货号：3211S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化Etk (Tyr40)的抗体仅在Etk的Tyr40位点磷酸化时能检测到内源Etk蛋白的表达。本抗体可能与激活的EGFR以及家族其它成员如高表达的ITK 和 BTK，发生交叉反应。通过用多肽免疫动物得到，该磷酸化多肽是根据人的Etk蛋白Tyr40附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。Etk,即BMX，是Bruton's 酪氨酸激酶(Btk)家族的成员之一（1）。它表达在各种造血细胞、上皮细胞和内皮细胞中。Etk和Btk家族的其它成员一样含有一个普列克底物蛋白同源性 (PH)区以及Src 同源性 SH3和 SH2区。它参与到多种信号转导途径（2）。Src激酶使Tyr566磷酸化对于体内Etk的活化是必须的（3）。在内皮细胞和上皮细胞中，FAK 通过Tyr40 位点的磷酸化调控Etk（4）。

1. Tamagnone, L. et al. (1994) Oncogene 9, 3683-8.
2. Qiu, Y. and Kung, H.J. (2000) Oncogene 19, 5651-61.
3. Tsai, Y.T. et al. (2000) Mol Cell Biol 20, 2043-54.
4. Chen, R. et al. (2001) Nat Cell Biol 3, 439-44.

原厂资料：

Specificity / Sensitivity

Phospho-Etk (Tyr40) Antibody detects endogenous levels of Etk only when phosphorylated at Tyr40. This antibody may cross react with activated EGF Receptor. This antibody may cross react with other family members including ITK and BTK when overexpressed.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr40 of human Etk. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Etk, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family (1). It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways (2). Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo (3). In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40 (4).

1. Tamagnone, L. et al. (1994) Oncogene 9, 3683-8.
2. Qiu, Y. and Kung, H.J. (2000) Oncogene 19, 5651-61.
3. Tsai, Y.T. et al. (2000) Mol Cell Biol 20, 2043-54.
4. Chen, R. et al. (2001) Nat Cell Biol 3, 439-44.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.