Mic-1 (L300) Antibody

• 产品编号：CST-3209S      品牌：CST       原厂货号：3209S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Mic-1 (L300)抗体识别内源性的总Mic-1 蛋白，包括他的活性加工形式.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Mic-1蛋白C末端的氨基酸序列一致，抗体由蛋白A和肽段亲和层析技术纯化.巨噬细胞抑制性细胞因子1(Mic-1)，也称GDF15 (1), PTGF-β (2), PLAB (3), PDF (4), 和NAG-1 (5)，是转化生长因子-β (TGF-β)超家族的成员（6）。就像其他家族成员，Mic-1合成为失活前体，这种前体经历酶解加工，涉及N末端疏水信号序列的去除，这种去除紧接着是在保守的RXXR位点剪切产生活性的C末端结构域，此结构域以二聚体蛋白形式分泌。Mic-1在胎盘中高表达，也在细胞压力，急性损伤，炎症和癌症中显著增加（7）。它也是p53肿瘤抑制蛋白的转录靶标，可能作为p53活性的生物标志物（8,9）。在肿瘤进程中，Mic-1对细胞凋亡，分化，血管生成和转移有多种效应，可能也对癌症中的病人体重下降有关（10,11）.

1. Strelau, J. et al. (2000) J Neurosci 20, 8597-603.
2. Yokoyama-Kobayashi, M. et al. (1997) J Biochem 122, 622-6.
3. Hromas, R. et al. (1997) Biochim Biophys Acta 1354, 40-4.
4. Paralkar, V.M. et al. (1998) J Biol Chem 273, 13760-7.
5. Baek, S.J. et al. (2001) J Biol Chem 276, 33384-92.
6. Bootcov, M.R. et al. (1997) Proc Natl Acad Sci USA 94, 11514-9.
7. Strelau, J. et al. (2000) J Neural Transm Suppl , 273-6.
8. Kannan, K. et al. (2000) FEBS Lett 470, 77-82.
9. Yang, H. et al. (2003) Mol Cancer Ther 2, 1023-9.
10. Johnen, H. et al. (2007) Nat Med 13, 1333-40.
11. Bauskin, A.R. et al. (2006) Cancer Res 66, 4983-6.

原厂资料：

Specificity / Sensitivity

Mic-1 (L300) Antibody detects endogenous levels of total Mic-1 protein including its active processed form.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human Mic-1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-β (2), PLAB (3), PDF (4), and NAG-1 (5) is a divergent member of the transforming growth factor-β (TGF-β) superfamily (6). Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site generating an active C-terminal domain that is secreted as a dimeric protein. Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer. In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7). It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9). During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenisis, and metastasis, and may also contribute to weight loss during cancer (10,11).

1. Strelau, J. et al. (2000) J Neurosci 20, 8597-603.
2. Yokoyama-Kobayashi, M. et al. (1997) J Biochem 122, 622-6.
3. Hromas, R. et al. (1997) Biochim Biophys Acta 1354, 40-4.
4. Paralkar, V.M. et al. (1998) J Biol Chem 273, 13760-7.
5. Baek, S.J. et al. (2001) J Biol Chem 276, 33384-92.
6. Bootcov, M.R. et al. (1997) Proc Natl Acad Sci USA 94, 11514-9.
7. Strelau, J. et al. (2000) J Neural Transm Suppl , 273-6.
8. Kannan, K. et al. (2000) FEBS Lett 470, 77-82.
9. Yang, H. et al. (2003) Mol Cancer Ther 2, 1023-9.
10. Johnen, H. et al. (2007) Nat Med 13, 1333-40.
11. Bauskin, A.R. et al. (2006) Cancer Res 66, 4983-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.