Yes Antibody

• 产品编号：CST-3201S      品牌：CST       原厂货号：3201S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Yes Antibody能够检测内源Yes蛋白总体水平。此抗体不与Src交叉反应。此多克隆抗体是通过合成人源对应的Yes N-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。细胞的致癌基因c-Yes及其病毒的同源基因 v-Yes (Yamaguchi 73的转换基因和Esh鸟肉瘤病毒) 编码一个60 kDa，存在于细胞质并与膜相关的酪氨酸激酶蛋白(1)。Yes 属于Src激酶家族并在很多细胞和组织中广泛的表达。与Src家族中的其它成员一样, Yes包含几个保守的功能区域，如N-末端靶标为细胞膜的肉豆蔻基序列、SH2 和 SH3 域、激酶域、C-末端无催化区域(2)。尽管有一些证据支持在Yes和Src激酶家族其它成员信号之间有冗余，但是也有相当的证据表明Yes信号通路有其独特的方面(2)。Yes能激活下游很多的细胞表面受体，包括酪氨酸激酶受体、G蛋白偶联受体、细胞因子受体(3)。另外，Yes和Src激酶都在细胞周期从G2期向M期转换过程中激活(3)。研究者发现Yes功能的紊乱与很多癌症的发展有关(4)。

1. Pena, S.V. et al. (1995) Gastroenterology 108, 117-24.
2. Summy, J.M. et al. (2003) Front Biosci 8, s185-205.
3. Summy, J.M. et al. (2003) J Cell Sci 116, 2585-98.
4. Rungsipipat, A. et al. (1999) Res Vet Sci 66, 205-10.

原厂资料：

Specificity / Sensitivity

Yes Antibody detects endogenous levels of total Yes protein. This antibody does not cross-react with Src.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Yes protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The cellular oncogene c-Yes and its viral homologue v-Yes (the transforming gene of Yamaguchi 73 and Esh avian sarcoma viruses) encode a 60 kDa, cytoplasmic, membrane-associated, protein-tyrosine kinase (1). Yes belongs to the Src kinase family and is ubiquitously expressed in many tissues and cells. Like other Src family members, Yes contains several conserved functional domains such as an N-terminal myristoylation sequence for membrane targeting, SH2 and SH3 domains, a kinase domain, and a C-terminal non-catalytic domain (2). Although several lines of evidence support redundancy in signaling between Yes and other Src family kinases, there is also a growing body of evidence indicating specificity in Yes signaling (2). Yes is activated downstream of a multitude of cell surface receptors, including receptor tyrosine kinases, G protein-coupled receptors, and cytokine receptors (3). In addition, both Yes and Src kinases are activated during the cell cycle transition from G2 to M phase (3). Investigators have found that dysfunction of Yes is associated with the development of various cancers (4).

1. Pena, S.V. et al. (1995) Gastroenterology 108, 117-24.
2. Summy, J.M. et al. (2003) Front Biosci 8, s185-205.
3. Summy, J.M. et al. (2003) J Cell Sci 116, 2585-98.
4. Rungsipipat, A. et al. (1999) Res Vet Sci 66, 205-10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.