SynGAP Antibody

• 产品编号：CST-3200S      品牌：CST       原厂货号：3200S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SynGAP Antibody 兔多抗识别内源性的SynGAP 总蛋白。该多克隆抗体由合成的人类SynGAP蛋白氨基酸序列对应肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。SynGAP是一个突触的GTP酶激活蛋白，在大脑中选择性地表达，目前发现其在哺乳动物脑兴奋性突触中浓度更高。SynGAP有一个PH结构域，一个C2的域名，和一个高度保守的RasGAP域，其负调控Ras活性和其下游的信号通路。SynGAP与SAP102的PDZ结构域相互作用，也可与PSD95相互作用，PSD95是一个突触后链接蛋白，可偶联SynGAP和NMDA受体（1）。SynGAP被Ca2+/钙调蛋白依赖的蛋白激酶II（CaMKII）在各个位点中的Ser765和Ser1123两处进行磷酸化。（2,3）。SynGAP的磷酸化激活GTP酶的Ras的活性，PSD95依赖的CaMKII磷酸化SynGAP在短暂性脑缺血后增加（1,4）。SynGAP与NMDA受体和CaMKII依赖AMPAR转运调节有关，在突触可塑性中扮演重要角色（3,5）。SynGAP对神经发育至关重要，缺乏SynGAP蛋白的小鼠在产后即死亡。此外，SynGAP突变小鼠的LTP降低，执行空间记忆任务也更加困难（6）。

1. Kim, J.H. et al. (1998) Neuron 20, 683-91.
2. Oh, J.S. et al. (2004) J Biol Chem 279, 17980-8.
3. Krapivinsky, G. et al. (2004) Neuron 43, 563-74.
4. Song, B. et al. (2004) Brain Res 1005, 44-50.
5. Komiyama, N.H. et al. (2002) J Neurosci 22, 9721-32.
6. Kim, J.H. et al. (2003) J Neurosci 23, 1119-24.

原厂资料：

Specificity / Sensitivity

SynGAP Antibody detects endogenous levels of total SynGAP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SynGAP. Antibodies are purified by peptide affinity chromatography.

Background

SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations specifically at excitatory synapses in the mammalian forebrain. SynGAP has a PH domain, a C2 domain, and a highly conserved RasGAP domain, which negatively regulates both Ras activity and its downstream signaling pathways. SynGAP interacts with the PDZ domains for SAP102, as well as PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (1). SynGAP is phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaMKII) at Ser765 and Ser1123 among other sites (2,3). Phosphorylation of SynGAP results in stimulation of GTPase activity of Ras, and PSD95 dependent CaMKII phosphorylation of SynGAP increases after transient brain ischemia (1,4). SynGAP is implicated in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking and plays an important role in synaptic plasticity (3,5). SynGAP is critical during neuronal development since mice lacking SynGAP protein die postnatally. Furthermore, SynGAP mutant mice have reduced LTP and perform poorly in spatial memory tasks (6).

1. Kim, J.H. et al. (1998) Neuron 20, 683-91.
2. Oh, J.S. et al. (2004) J Biol Chem 279, 17980-8.
3. Krapivinsky, G. et al. (2004) Neuron 43, 563-74.
4. Song, B. et al. (2004) Brain Res 1005, 44-50.
5. Komiyama, N.H. et al. (2002) J Neurosci 22, 9721-32.
6. Kim, J.H. et al. (2003) J Neurosci 23, 1119-24.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.