Fatty Acid Synthase Antibody

• 产品编号：CST-3189S      品牌：CST       原厂货号：3189S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fatty Acid Synthase抗体识别内源性的脂肪酸合成酶总蛋白水平。该多克隆抗体用与小鼠脂肪酸合成酶Ala1160位点附近氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。脂肪酸合成酶(FAS)催化乙酰辅酶A和丙二酰辅酶A合成长链脂肪酸。FAS以同源二聚体发挥作用，有7种不同的催化活性，可以在肝脏中制造脂质以便输送到有代谢活性的组织或脂肪储存组织。在大多数人类组织中，FAS都以最小量表达，因为它们需要循环利用脂肪酸以制造新的结构脂质（1）。最近发现，FAS表达增加似乎是人类恶性肿瘤中的一个常见表型。在乳腺癌中，免疫组织化学染色发现FAS的水平与乳腺癌体积直接相关（2）。研究还发现FAS在肺癌和前列腺癌中高表达，FAS的表达是乳腺癌和前列腺癌预后不良的指标（3-5）。另外，抑制FAS的表达对人类癌细胞有选择性的细胞毒作用（5）。因此，FAS吸引了越来越多的研究者兴趣，它除了在代谢综合症中发挥作用外，它可能同时蕴含着在肿瘤的诊断和治疗中的潜在作用（6,7）。

1. Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
2. Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40.
3. Kawamura, T. et al. (2005) Pathobiology 72, 233-240.
4. Shah, U.S. et al. (2006) Hum Pathol 37, 401-409.
5. Kuhajda, F.P. (2000) Nutrition 16, 202-8.
6. Tian, W.X. (2006) Curr Med Chem 13, 967-977.
7. Kusunoki, J. et al. (2006) Endocrine 29, 91-100.

原厂资料：

Specificity / Sensitivity

Fatty Acid Synthase Antibody detects endogenous levels of total fatty acid synthase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide around Ala1160 corresponding to a sequence of mouse fatty acid synthase. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Fatty acid synthase (FAS) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FAS is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FAS is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1). 
 More recently, increased expression of FAS has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FAS are directly related to the size of breast tumors (2). Studies also showed that FAS is highly expressed in lung and prostate cancers and that FAS expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FAS is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FAS as a potential target for the diagnosis and treatment of cancer as well as the metabolic syndrome (6,7).

1. Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
2. Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40.
3. Kawamura, T. et al. (2005) Pathobiology 72, 233-240.
4. Shah, U.S. et al. (2006) Hum Pathol 37, 401-409.
5. Kuhajda, F.P. (2000) Nutrition 16, 202-8.
6. Tian, W.X. (2006) Curr Med Chem 13, 967-977.
7. Kusunoki, J. et al. (2006) Endocrine 29, 91-100.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.