Phospho-PERK (Thr980) (16F8) Rabbit mAb

• 产品编号：CST-3179S      品牌：CST       原厂货号：3179S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PERK (Thr980) (16F8) Rabbit mAb兔单抗可识别内源性的Thr980磷酸化的PERK。该单克隆抗体用与小鼠PERK蛋白中Thr980位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物而制成。蛋白激酶类似的内质网激酶(PERK)是一种eIF2α激酶并且为一种定位在endoplasmic reticulum (ER) 膜上的跨膜蛋白，能够传导ER应激信号从而抑制翻译（1-3）。ER应激会提高PERK的活性，然后使eIF2α磷酸化从而增加翻译水平的降低。研究证实PERK缺失的小鼠在出生后的几周内其胰岛β细胞会出现缺陷，这也暗示着PERK介导的翻译控制在ER应激时保护分泌细胞的重要作用（4）。ER应激时PERK的活化与其胞内激酶结构域的自身磷酸化相关联（1-3）。PERK Thr980位点的磷酸化是其自身活化状态的一个标志。

1. Harding, H. et al. (1999) Nature 397, 271-274.
2. Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
3. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

原厂资料：

Specificity / Sensitivity

Phospho-PERK (Thr980) (16F8) Rabbit mAb detects endogenous levels of PERK phosphorylated at Thr980.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr980 of mouse PERK.

Background

PERK (protein kinase-like endoplasmic reticulum kinase) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.

1. Harding, H. et al. (1999) Nature 397, 271-274.
2. Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.
3. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.