BiP (C50B12) Rabbit mAb

• 产品编号：CST-3177P      品牌：Cell Signaling Technology       原厂货号：3177P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BiP (C50B12) Rabbit mAb识别内源性的BiP总蛋白。BiP (C50B12) Rabbit mAb兔单抗用与人类BiP蛋白Gly584附近氨基酸序列对应的人工合成肽段免疫兔而制成。分泌型和跨膜型蛋白以多核糖体形式合成，然后转移到内质网中。在内质网中，这些蛋白进行常见的二硫键形成、氨基糖基化和折叠。为了帮助蛋白质正确折叠，内质网包含一些列分子伴侣，包括BiP。BiP是被鉴定为一种前B细胞中免疫球蛋白重链结合蛋白（1，2）。也发现在葡萄糖饥饿时其可在蛋白质水平被诱导（3）。如果内质网内蛋白折叠被扰乱，Bip合成会增加。接着，BiP结合到错误的蛋白质上，防止其形成聚集物，并帮助其重新正确折叠（4）。

1. Wabl, M. and Steinberg, C. (1982) Proc Natl Acad Sci U S A 79, 6976-8.
2. Haas, I.G. and Wabl, M. (1983) Nature 306, 387-9.
3. Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300.
4. Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90.

原厂资料：

Specificity / Sensitivity

BiP (C50B12) Rabbit mAb detects endogenous levels of total BiP protein.

Source / Purification

BiP (C50B12) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gly584 of human BiP.

Background

Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).

1. Wabl, M. and Steinberg, C. (1982) Proc Natl Acad Sci U S A 79, 6976-8.
2. Haas, I.G. and Wabl, M. (1983) Nature 306, 387-9.
3. Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300.
4. Kohno, K. et al. (1993) Mol Cell Biol 13, 877-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.