RNF40 Antibody

• 产品编号：CST-3167S      品牌：Cell Signaling Technology       原厂货号：3167S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RNF40 Antibody能够检测内源性RNF40总蛋白水平。该抗体不与RNF20蛋白发生交叉反应。通过合成的人源RNF40蛋白Pro610位点周围相应的多肽去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。哺乳动物细胞中，染色质表观遗传学中histone H2B泛素化的意义来自出芽酵母蛋白Bre1的鉴定(1,2)。与泛素连接酶Rad6一起，Bre1蛋白在染色质的转录区域里酵母histone H2B的单泛素化中作为E3连接酶(1-3)。随后，酵母Bre1蛋白的哺乳动物直系同源RNF20和RNF40蛋白被鉴定(4,5)。这两个蛋白质形成一个紧密异源二聚体，该酶作为主要E3连接酶负责哺乳细胞中histone H2B蛋白Lys120位点单泛素化，该位点的修饰是在非损伤细胞中联系着RNA Pol II依赖的转录延伸。最近，研究显示DNA double-strand breaks (DSBs)也有能力诱导H2B的单泛素化。与ATM依赖的RNF20-RNF40异源二聚体的磷酸化一样，这个过程取决于招募到DSB位点，因此在DSB修复通路中增强对于E3连接酶的一个作用 (6)。的确，研究显示RNF20-RNF40功能的减少促进复制应激和染色体不稳定，这可能是细胞侵袭之前的恶性转化的早期步骤(7)。

1. Wood, A. et al. (2003) Mol Cell 11, 267-74.
2. Hwang, W.W. et al. (2003) Mol Cell 11, 261-6.
3. Kao, C.F. et al. (2004) Genes Dev 18, 184-95.
4. Kim, J. et al. (2005) Mol Cell 20, 759-70.
5. Zhu, B. et al. (2005) Mol Cell 20, 601-11.
6. Moyal, L. et al. (2011) Mol Cell 41, 529-42.
7. Chernikova, S.B. et al. (2012) Cancer Res, Epub ahead of print.

原厂资料：

Specificity / Sensitivity

RNF40 Antibody recognizes endogenous levels of total RNF40 protein. This antibody does not cross-react with RNF20.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro610 of human RNF40 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1 (1,2). Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin (1-3). Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified (4,5). These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Recently, it was shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways (6). Indeed, loss of RNF20-RNF40 function has been shown to promote replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion (7).

1. Wood, A. et al. (2003) Mol Cell 11, 267-74.
2. Hwang, W.W. et al. (2003) Mol Cell 11, 261-6.
3. Kao, C.F. et al. (2004) Genes Dev 18, 184-95.
4. Kim, J. et al. (2005) Mol Cell 20, 759-70.
5. Zhu, B. et al. (2005) Mol Cell 20, 601-11.
6. Moyal, L. et al. (2011) Mol Cell 41, 529-42.
7. Chernikova, S.B. et al. (2012) Cancer Res, Epub ahead of print.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.