c-IAP2 (58C7) Rabbit mAb

• 产品编号：CST-3130P      品牌：Cell Signaling Technology       原厂货号：3130P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

c-IAP2 (58C7) Rabbit mAb 兔单抗能够检测内源性水平c-IAP2总蛋白。该抗体不会与其他家族成员产生交叉反应。该单克隆抗体是采用合成的人源c-IAP2蛋白Val117周围残基相对应的肽段免疫动物而制备的。凋亡抑制因子蛋白(IAP) 家族包含一个进化上保守的凋亡抑制分支，此分支蛋白包含70个氨基酸的BIR (杆状病毒抑制重复) 域(1,2)。在人类中，此家族的蛋白包含c-IAP1、c-IAP2、XIAP、survivin、livin和NAIP。在癌细胞系和原癌中，IAP 家族成员过表达，尤其是survivin和livin, 这显示出了这些蛋白在癌症发展过程中的重要作用(3-5)。一般来讲，IAP蛋白发挥作用是通过直接抑制几个caspases的活性, 包括caspase-3、caspase-7和caspase-9 (5,6)。另外，IAP家族的成员与线粒体蛋白Smac的结合阻止了它们与caspase-9的互作, 因此导致了caspase的加工及其激活 (2)。

1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.

原厂资料：

Specificity / Sensitivity

c-IAP2 (58C7) Rabbit mAb detects endogenous levels of total c-IAP2 protein. No cross reactivity was detected with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val117 of human c-IAP2.

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).

1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
2. Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23.
3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.