IFN-α (8C21) Mouse mAb

• 产品编号：CST-3115S      品牌：CST       原厂货号：3115S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IFN-α (8C21) Mouse mAb能够检测重组人的干扰素α蛋白。此抗体不与人的干扰素-β 和 -γ交叉反应。单克隆抗体是通过提纯自然状态下人干扰素源α蛋白来免疫动物而获得。干扰素(IFNs)即是局部的又是系统性的，在受到病毒感染和参与限制干扰扩散过程中是系统性的。他们也能影响细胞的分化、生长、表面抗原的表达和免疫控制(1)。共有三种自然产生的干扰素: α, β 和 γ。IFN-α起源于成淋巴细胞组织并且在治疗各种人类肿瘤和病毒引起的疾病中有治疗应用价值。重组的IFN-α不管是自然发生的还是合成的基因，都能结合到一系列的细胞种类表面受体并诱导抗病毒的活性。当结合到目标细胞不相关的表面受体上时，IFN-α能够快速的诱导Jak/Stat磷酸化的改变，并起始Jak/Stat信号通路 (2)。IFN-α信号也参与了DAG的产生并且没有胞内游离钙离子浓度的增加和随后的钙依赖形式PKC (β和ε)的激活(3)。所有的IFN-α 信号通路最终导致了基因表达的改变并介导了它们多向性的生物活性。

1. Stiehm, E.R. et al. (1982) Ann Intern Med 96, 80-93.
2. Pellegrini, S. et al. (1989) Mol Cell Biol 9, 4605-12.
3. Pfeffer, L.M. and Colamonici, O.R. (1991) Pharmacol Ther 52, 149-57.

原厂资料：

Specificity / Sensitivity

IFN-α (8C21) Mouse mAb detects recombinant human interferon-α protein. This antibody does not cross-react with human interferon-β and -γ.

Source / Purification

Monoclonal antibody is produced by immunizing animals with purified natural human interferon-α proteins.

Background

Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression and immunoregulation (1). There are three naturally occurring interferons: α, β and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2). IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε) (3). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.

1. Stiehm, E.R. et al. (1982) Ann Intern Med 96, 80-93.
2. Pellegrini, S. et al. (1989) Mol Cell Biol 9, 4605-12.
3. Pfeffer, L.M. and Colamonici, O.R. (1991) Pharmacol Ther 52, 149-57.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.