Phospho-VASP (Ser239) Antibody

• 产品编号：CST-3114P      品牌：Cell Signaling Technology       原厂货号：3114P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-VASP (Ser239) Antibody检测仅在serine 239位点磷酸化的内源性VASP蛋白。该抗体不能与磷酸化的VASP同系物例如Mena蛋白发生交叉反应。通过人工合成人源VASP蛋白Ser239位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Vasodilator-stimulated phosphoprotein (VASP)起初的特征是分别作为cGMP- 和cAMP-依赖的激酶(PKG和PKA或cGPK 和cAPK)的一个底物(1)。研究认为VASP蛋白属于受体蛋白的Ena/VASP家族，而受体蛋白是可将细胞骨架系统联系到信号转导通路，并且其在细胞骨架的组织、成纤维细胞迁移、血小板活化和轴突导向中有一定作用(2,3)。三个磷酸化位点Ser157、Ser239和Thr278已经被鉴定。Ser239位点是主要的PKG磷酸化位点，而Ser157位点是主要的PKA磷酸化位点(4)。研究证明VASP磷酸化可减少它与actin的联系，并且有一个负性影响在actin的聚合作用 (5)。VASP蛋白Ser239位点的磷酸化是对监控PKG激活和信号发送起到一个很好的标记物的作用(6,7)。

1. Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
2. Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
3. Machesky, L.M. et al. (2000) Cell 101, 685-688.
4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
5. Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
6. Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
7. Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.

原厂资料：

Specificity / Sensitivity

Phospho-VASP (Ser239) Antibody detects endogenous levels of VASP only when phosphorylated at serine 239. The antibody does not cross-react with phosphorylated VASP homologues, such as Mena.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser239 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

1. Butt, E. et al. (1994) J. Biol. Chem. 269, 14509-14517.
2. Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
3. Machesky, L.M. et al. (2000) Cell 101, 685-688.
4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
5. Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817-30825.
6. Oelze, M. et al. (2000) Circ. Res. 87, 999-1005.
7. Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550-5556.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.