Aurora B/AIM1 Antibody

• 产品编号：CST-3094S      品牌：CST       原厂货号：3094S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Aurora B/AIM1 Antibody能够检测内源性的Aurora B/AIM1蛋白。该多克隆抗体是由合成的人源的针对Aurora B/AIM1氨基末端的肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。极光激酶A(AIK)是一种调节细胞周期的丝氨酸/苏氨酸蛋白激酶，在多种肿瘤细胞系中过表达(1-3)。在蛋白激酶激活回路中，极光激酶A苏氨酸(288位)的磷酸化可显著提高其活性并靶向有丝分裂过程中蛋白质的蛋白酶体降解(4)。与极光激酶A密切相关的极光激酶B(AIM1)在多重有丝分裂事件中发挥作用，siRNA沉默其表达之后可以导致组蛋白H3磷酸化减少、异常的染色体排列/分离以及生存蛋白的定位改变(6)。

1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

原厂资料：

Specificity / Sensitivity

Aurora B/AIM1 Antibody detects endogenous levels of Aurora B/AIM1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human Aurora B/AIM1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6).

1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.