IRS-2 (L1326) Antibody

• 产品编号：CST-3089S      品牌：CST       原厂货号：3089S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IRS-2 (L1326)抗体识别内源性的IRS-2总蛋白水平。此抗体不与IRS-1发生交叉反应。该多克隆抗体用与人类IRS-2中Leu1326位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。胰岛素受体底物2（IRS-2）是胰岛素受体激酶的主要底物之一（1）。在脊椎动物中，IRS-2以脚手架蛋白发挥作用，协调胰岛素/IGF信号级联通路中的不同分支（2）。IRS-2对正常营养动态平衡至关重要，因为它能同时介导外周胰岛素的作用和B-细胞中IGF-1的作用。缺乏IRS-2的小鼠不能保持足够的胰岛素代偿分泌，容易在青壮年时发生糖尿病（3）。

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

原厂资料：

Specificity / Sensitivity

IRS-2 (L1326) Antibody detects endogenous levels of total IRS-2 protein. It does not cross-react with IRS-1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1326 of human IRS-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.