HSPB8/HSP22 Antibody

• 产品编号：CST-3059S      品牌：CST       原厂货号：3059S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HSPB8/HSP22 Antibody识别内源性的HSPB8/HSP22总蛋白。该多克隆抗体用与人类HSPB8/HSP22蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。HSPB8（HSP22）是小热休克蛋白超家族的成员，该人类蛋白与HSP27最为密切相关。与大多数其它小热休克蛋白HSP（sHSPs）类似，HSPB8主要在骨骼和心脏中转录（1）。在一个双杂交筛选中，HSPB8主要与一种三天门冬氨酸形式的HSP27相互作用，与野生型HSP27相比，其模拟了HSP27在Ser15、Ser78和Ser82的磷酸化（2）。HSPB8有两个结合区域（N端和C端），可特异性的结合不同伙伴，具有自身结合能力以及结合其它sHSPs如HSPB7和HSPB2de能力（3）。分子伴侣类似的活性对HSP22在多个不同过程中发挥作用具有特别的重要性，这些过程包括增殖、凋亡和大自噬（4）。

1. Kappé, G. et al. (2001) Biochim Biophys Acta 1520, 1-6.
2. Benndorf, R. et al. (2001) J Biol Chem 276, 26753-61.
3. Sun, X. et al. (2004) J Biol Chem 279, 2394-402.
4. Kim, M.V. et al. (2004) Biochem Biophys Res Commun 325, 649-52.

原厂资料：

Specificity / Sensitivity

HSPB8/HSP22 Antibody detects endogenous levels of total HSPB8/HSP22 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSPB8/HSP22. Antibodies are purified by protein A and peptide affinity chromatography.

Background

HSPB8 (HSP22) is a member of the small heat shock protein superfamily and the human protein is most closely related to HSP27. Similar to most other small HSPs (sHSPs), HSPB8 is predominantly transcribed in skeletal muscle and heart (1). In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N- and C-terminal) that are specific for different binding partners, and has the ability to bind to itself and other sHSPs such as HSPB7 and HSPB2 (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macroautophagy (4).

1. Kappé, G. et al. (2001) Biochim Biophys Acta 1520, 1-6.
2. Benndorf, R. et al. (2001) J Biol Chem 276, 26753-61.
3. Sun, X. et al. (2004) J Biol Chem 279, 2394-402.
4. Kim, M.V. et al. (2004) Biochem Biophys Res Commun 325, 649-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.