DUSP6/MKP3 Antibody

• 产品编号：CST-3058S      品牌：CST       原厂货号：3058S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MKP3 Antibody兔多抗能检测内源性总MKP3蛋白水平。该多克隆抗体通过使用与人源MKP3蛋白序列相一致的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。MAPK能被双特异性蛋白磷酸酶（DUSPs）失活，DUSPs由其不同的底物、组织分布、细胞外刺激的诱导性和细胞内定位而不同。DUSPs，也被称为MAPK磷酸酶（MKP），能够特异性的去磷酸化MAPK P-loops的苏氨酸和酪氨酸残基，在调控MAPK家族功能的过程中发挥了重要作用（1,2）。已经发现了至少13个成员(MKP-1, PAC-1, VHR, MKP-2, HVH3, MKP-3, PYST2, HVH5, MKP-4, MKP-5, MKP-6, MKP-7, DSP2)，针对多种MAPK展现了独特的底物特异性（3）。MAPK磷酸酶含有典型的氨基端rhodanese-fold以便DUSP与MAPK家族成员结合，和一个羧基端催化结构域（4）。这些磷酸酶在发育、免疫系统功能、压力应激和代谢稳态过程中发挥了重要作用（5）。此外，研究提示DUSPs涉及了癌症发展和癌细胞对化疗的反应（6）。

1. Camps, M. et al. (2000) FASEB J 14, 6-16.
2. Theodosiou, A. and Ashworth, A. (2002) Genome Biol 3, REVIEWS3009.
3. Salojin, K. and Oravecz, T. (2007) J Leukoc Biol 81, 860-9.
4. Tanoue, T. et al. (2002) J Biol Chem 277, 22942-9.
5. Dickinson, R.J. and Keyse, S.M. (2006) J Cell Sci 119, 4607-15.
6. Wu, G.S. (2007) Cancer Metastasis Rev 26, 579-85.
7. Kim, Y. et al. (2003) Biochemistry 42, 15197-207.

原厂资料：

Specificity / Sensitivity

MKP3 Antibody detects endogenous levels of total MKP3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MKP3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MAP kinases are inactivated by dual-specificity protein phosphatases known as MAPK phosphatases (MKP), that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. MKPs specifically dephosphorylate both threonine and tyrosine residues in MAPK P-loops and have been shown to play important roles in regulating the function of the MAPK family (1,2). At least 13 members of the family (MKP-1, PAC-1, VHR, MKP-2, HVH3, MKP-3, PYST2, HVH5, MKP-4, MKP-5, MKP-6, MKP-7, DSP2) display unique substrate specificities for various MAP kinases (3). MAPK phosphatases typically contain an amino-terminal rhodanese-fold responsible for MKP docking to MAPK family members and a carboxy-terminal catalytic domain (4). These phosphatases can play important roles in development, immune system function, stress responses and metabolic homeostasis (5), and also in the development of cancer and the response of cancer cells to chemotherapy (6).

1. Camps, M. et al. (2000) FASEB J 14, 6-16.
2. Theodosiou, A. and Ashworth, A. (2002) Genome Biol 3, REVIEWS3009.
3. Salojin, K. and Oravecz, T. (2007) J Leukoc Biol 81, 860-9.
4. Tanoue, T. et al. (2002) J Biol Chem 277, 22942-9.
5. Dickinson, R.J. and Keyse, S.M. (2006) J Cell Sci 119, 4607-15.
6. Wu, G.S. (2007) Cancer Metastasis Rev 26, 579-85.
7. Kim, Y. et al. (2003) Biochemistry 42, 15197-207.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.