Phospho-LKB1 (Thr189) Antibody

• 产品编号：CST-3054S      品牌：CST       原厂货号：3054S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-LKB1 (Thr189)抗体识别内源性的Thr189磷酸化的LKB1蛋白。此抗体与其它位点磷酸化的LKB1蛋白没有交叉反应。该多克隆抗体用与人类LKB1中Thr189位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。LKB1（STK11）是一个丝氨酸/苏氨酸激酶，也是一个肿瘤抑制因子，可以通过对多种下游激酶的调控而控制细胞结构、凋亡和能量平衡（1,2）。LKB1、一种推测激酶STRAD和MO25脚手架蛋白组成胞质蛋白复合物，它可以激活AMP-激活的蛋白激酶（AMPK）和几种AMPK-相关激酶（3）。AMPK是细胞能量平衡的主要调节者，控制下游效应器，从而调节对ATP浓度反应的细胞生长、凋亡（4）。LKB1似乎可以在细胞内在多个位点被磷酸化，包括人LKB1的Ser31/325/428和Thr189/336/363（5）。LKB1基因的相应变异会导致Peutz-Jeghers综合征（PJS），这是一宗常染色体显性的疾病，以良性胃肠道息肉和口、手、足处皮肤深色变为特点（6）。LKB1的其它一些突变与若干组织中的散发癌症的形成有关（7）。

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

原厂资料：

Specificity / Sensitivity

Phospho-LKB1 (Thr189) Antibody detects transfected levels of LKB1 only when phosphorylated at threonine 189. The antibody does not cross-react with LKB1 phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr189 of human LKB1. Antibodies are purified by protein A and peptide affinity chromatography

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, 
including human LKB1 at Ser31/325/428 and Thr189/336/363 (5). 
 Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.