Met Signaling Antibody Sampler Kit

• 产品编号：CST-3019S      品牌：Cell Signaling Technology       原厂货号：3019S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Met Signaling Antibody Sampler Kit中的每一种抗体能够检测其特异的靶分子内源性的表达水平但不与家族中的其它成员发生交叉反应，特别说明者除外。磷酸化Met (Tyr1234/1235) (D26) 兔单抗 可能在免疫印迹检测中与过表达的酪氨酸酶磷酸化的Src蛋白发生交叉反应。磷酸化Met (Tyr1349) (130H2) 兔单抗 可能与其它激活的酪氨酸激酶蛋白发生交叉反应。磷酸化Met (Tyr1003) (13D11) 兔单抗可能与与其它激活的酪氨酸激酶蛋白发生交叉反应。磷酸化Gab1 (Tyr307) 抗体 与磷酸化的Gab2和磷酸化的Gab3发生交叉反应。单克隆抗体通过免疫动物得到，该磷酸化多肽或多肽是根据人的Met蛋白Tyr1003, Tyr1234/1235, Tyr1349, 或 Tyr1234附近的氨基酸序列合成的。多克隆抗体通过用多肽免疫动物得到，该磷酸化多肽或多肽是根据人的Gab1蛋白Tyr472 和Tyr307附近的氨基酸序列合成的。多克隆抗体经过protein A和亲和层析纯化。Met Signaling Antibody Sampler Kit是检测特异位点磷酸化的Met信号的快速、经济的选择。本试剂盒的一抗和二抗足够四次免疫印记检测实验使用Met作为肝细胞生长因子 (HGF, 亦作扩散因子) 的高亲和性酪氨酸激酶受体是一个二硫键连接的异二聚体，含有一个45 kDa的 α-亚基和一个145 kDa的 β-亚基 (1,2)。α-亚基和β-亚基的氨基端形成胞外区，β-亚基的其余部分跨细胞膜并且含有一个具有激酶活性的胞内区。Met和HGF相互作用会导致多个酪氨酸位点的自磷酸化，继而招募下游信号分子Gab1, c-Cbl和 PI3 K激酶（3）。这一系列基础性事件对于Met激酶相关的所有生物学功能十分重要。胞内Tyr1003位点的磷酸化对于Met的泛素化和降解是必须的（4）。激酶结构域Tyr1234/1235的磷酸化对于Met的激酶活性十分关键。胞内 Tyr1349的磷酸化为Met提供了Gab1 的直接结合位点(5)。在肾、结肠和乳腺癌中发现有Met水平和/或激酶活性的改变，因此Met是肿瘤治疗和诊断的一个靶点（6,7）。

原厂资料：

Specificity / Sensitivity

Each antibody in the Met Signaling Antibody Sampler Kit recognizes endogenous levels of its specific target and does not cross-react with other family members unless otherwise indicated. Phospho-Met (Tyr1234/1235) (D26) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated Src proteins in Western blot. Phospho-Met (Tyr1349) (130H2) Rabbit mAb may cross-react with other activated protein tyrosine kinases. Phospho-Met (Tyr1003) (13D11) Rabbit mAb may cross-react with other activated protein tyrosine kinases. Phospho-Gab1 (Tyr307) Antibody cross-reacts with phosphorylated Gab2 and potentially with phosphorylated Gab3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phosphopeptides or peptides corresponding to residues surrounding: Tyr1003, Tyr1234/1235, Tyr1349, or Tyr1234 of human Met. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide or peptide corresponding to residues surrounding Tyr472 and Tyr307 of human Gab1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Met Signaling Antibody Sampler Kit provides an economical means to investigate Met signaling. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.

Background

Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor), is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. Addition of a phosphate at cytoplasmic Tyr1003 is essential for ubiquitination and Met protein degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast cancers. Thus, Met is an attractive cancer therapeutic and diagnostic target (6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodies.