Insulin Receptor Substrate Antibody Sampler Kit

• 产品编号：CST-3015S      品牌：Cell Signaling Technology       原厂货号：3015S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Phospho-IRS-1 (Ser307)抗体、Phospho-IRS-1 (Ser612) (C15H5)兔单抗和Phospho-IRS-1 (Ser318)抗体分别识别内源性的Ser307、Ser612或者Ser318磷酸化的IRS-1蛋白水平。注意：Ser318是小鼠大氨基酸残基，对应的人类氨基酸残基为Ser323。·IRS-1抗体和IRS-2抗体分别识别内源性的IRS-1或IRS-2总蛋白水平。Phospho-IRS-1 (Ser318)抗体也可以识别一条诱导的非特异的57kDa条带。该多克隆抗体用与人类IRS-2中羧基末端的氨基酸序列和小鼠IRS-1的Ser307（与人IRS-1 Ser312同源）附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。该单克隆抗体用与小鼠IRS-1中Ser612位点、人类IRS-1中Ser270位点或小鼠IRS-1中Ser318附近的氨基酸序列对应的人工合成肽段免疫动物制成。Insulin Receptor Substrate Antibody Sampler Kit提供了一个经济的手段来研究IRS-1和IRS-2信号通路及其在细胞内的磷酸化情况。该试剂盒包含足量的每个一抗进行四次Western blots的抗体。胰岛素受体底物1（IRS-1）是胰岛素受体激酶的主要底物之一（1）。IRS-1包括多个酪氨酸磷酸化模体，作为与包含SH2结构域的蛋白质的接合位点，介导胰岛素的代谢和生长促进功能（2-4）。IRS-1也包含超过30个潜在的丝氨酸/苏氨酸磷酸化位点。JNK（5）和IKK（6）对IRS-1 Ser307位点进行磷酸化，SIK2（AMPK家族成员之一）对Ser789进行磷酸化（7）。PKC和mTOR途径分别介导IRS-1的Ser612和Ser636/639磷酸化（8,9）。IRS-1在Ser1101的磷酸化由PKCθ介导，可以导致细胞内胰岛素信号通路的抑制，这暗示其在某些肥胖模型中的胰岛素抵抗机制中发挥作用（10）。

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
3. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
4. Wang, L.M. et al. (1993) Science 261, 1591-1594.
5. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
6. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
8. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
9. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
10. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

原厂资料：

Specificity / Sensitivity

Phospho-IRS-1 (Ser307) Antibody, Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb and Phospho-IRS-1 (Ser318) Antibody detect endogenous levels of IRS-1 only when phosphorylated at Ser307, Ser612 or Ser318, respectively. Note: Ser318 is the mouse residue; the corresponding human residue is Ser323. IRS-1 Antibody and IRS-2 Antibody detect endogenous levels of total IRS-1 or IRS-2, respectively. The Phospho-IRS-1 (Ser318) antibody cross reacts with a inducible nonspecific band at around 57 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human IRS-2 and residues surrounding Ser307 of mouse IRS-1 (homologous to Ser312 of human IRS-1). Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser612 of mouse IRS-1, a synthetic peptide corresponding to the sequence surrounding Ser270 of human IRS-1 and a synthetic phosphopeptide corresponding to residues surrounding Ser318 of mouse IRS-1 protein.

Description

The Insulin Receptor Substrate Antibody Sampler Kit provides an economical means to investigate IRS-1 and IRS-2 signaling and phosphorylation within the cell. The kit contains enough antibody to perform four western blots with each primary antibody.

Background

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
3. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
4. Wang, L.M. et al. (1993) Science 261, 1591-1594.
5. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
6. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
8. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
9. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
10. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibodies