TBK1/NAK Antibody

• 产品编号：CST-3013S      品牌：CST       原厂货号：3013S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TBK1 抗体能够检测内源TBK1/NAK 蛋白总体水平。此多克隆抗体是通过合成人源对应的TBK1/NAK C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。TBK1 (TANK-结合激酶1)/NAK (NF-κB 激活激酶)是IκB激酶(IKK)的激活性激酶，能够通过直接磷酸化激活IKK(1)。 TBK1的鉴定是通过与之相关的TRAF结合蛋白——TANK, 并发现在NF-κB的激活中在NIK和IKK 的上游起作用(2)。TBK1/NAK 诱导IκB 的降解和通过IKKβ而激活 NF-κB。生长激素刺激PKCε的活性，NAK 可能是对此作出应答并介导IKK 和 NF-κB的激活(1)。TBK1在天然免疫激活IRF3的过程中发挥了重要的作用(3)。

1. Tojima, Y. et al. (2000) Nature 404, 778-82.
2. Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
3. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.

原厂资料：

Specificity / Sensitivity

TBK1 Antibody detects endogenous levels of total TBK1/NAK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of TBK1/NAK. Antibodies were purified by protein A and peptide affinity chromatography.

Background

TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1/NAK induces IκB degradation and NF-κB activity through IKKβ. NAK may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).

1. Tojima, Y. et al. (2000) Nature 404, 778-82.
2. Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
3. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.