PI3 Kinase p110β (C33D4) Rabbit mAb

• 产品编号：CST-3011S      品牌：CST       原厂货号：3011S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PI3 Kinase p110β (C33D4) Rabbit mAb兔单抗能够检测内源性的PI3K p110β 总蛋白。该单克隆抗体是用合成的人源的PI3K p110β 肽段免疫动物获得的。PI3K激酶通过磷酸化磷脂酰肌醇(PI), 4-磷酸磷脂酰肌醇 (PIP)和4，5-二磷酸磷脂酰肌醇 (PIP2)催化3,4,5-三磷酸磷脂酰肌醇的产生。生长因子和激素会引发该磷酸化进程，随后协助细胞生长，细胞周期进入，细胞迁移和细胞存活（1）。PTEN会逆转该过程，研究表明PI3K信号通路在PTEN功能缺失的人类癌症中是持续性激活的（2）。PI3Ks由一个催化亚基（p110）和一个调控亚基构成。已经分离得到了多个催化亚基(p110α, p110β, p110γ, 和p110δ)，与p110α, p110β, 和 p110δ结合的调控亚基是p85α 和 p85β (3).相对的，与p101调控亚基结合的p110γ与p85无关。此外，异源三聚体G蛋白的βγ亚基可以激活p110γ(4)。 p110β广泛分布于各种组织并且在胚胎早期发育过程中起关键作用（5）。p110β激活细胞增殖，侵袭性细胞生长，而且在一系列包括胶质母细胞瘤等肿瘤中的表达量增加（6-8）。

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
4. Stoyanov, B. et al. (1995) Science 269, 690-3.
5. Okkenhaug, K. and Vanhaesebroeck, B. (2003) Nat Rev Immunol 3, 317-30.
6. Czauderna, F. et al. (2003) Nucleic Acids Res 31, 670-82.
7. Bénistant, C. et al. (2000) Oncogene 19, 5083-90.
8. Knobbe, C.B. and Reifenberger, G. (2003) Brain Pathol 13, 507-18.

原厂资料：

Specificity / Sensitivity

PI3 Kinase p110β (C33D4) Rabbit mAb detects endogenous levels of total PI3K p110β protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide of human PI3K p110β.

Background

Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).p110ß is widely distributed in tissue and plays an essential role in early embryonic development (5). p110ß stimulates cell proliferation, invasive cell growth, and expression is increased in a number of tumors including glioblastomas (6-8).

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
4. Stoyanov, B. et al. (1995) Science 269, 690-3.
5. Okkenhaug, K. and Vanhaesebroeck, B. (2003) Nat Rev Immunol 3, 317-30.
6. Czauderna, F. et al. (2003) Nucleic Acids Res 31, 670-82.
7. Bénistant, C. et al. (2000) Oncogene 19, 5083-90.
8. Knobbe, C.B. and Reifenberger, G. (2003) Brain Pathol 13, 507-18.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.