Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb

• 产品编号：CST-3007S      品牌：CST       原厂货号：3007S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb兔单抗能检测内源性Thr334位点磷酸化的MAPKAPK-2蛋白水平。该单克隆抗体是采用合成的与人源MAPKAPK-2蛋白Thr334位点周围残基相一致的磷酸化肽段免疫动物后获得的。

在细胞因子、压力和化学因子的刺激下，丝裂原活化蛋白激酶激活的蛋白激酶2(MAPKAPK-2)能够迅速被磷酸化并被激活。研究显示MAPKAPK-2是p38 MAPK的直接靶点（1）。在体内，在压力应激下MAPKAPK-2的多个氨基酸都能被磷酸化。但是，在体外激酶实验中，仅有4个位点(Thr25, Thr222, Ser272, 和 Thr334)能够被p38 MAPK磷酸化（2）。Thr222, Ser272和Thr334的磷酸化对于MAPKAPK-2的激酶活性是必须的（2）。Thr25可以在体外被p42 MAPK磷酸化，但是该磷酸化对于MAPKAPK-2的激活不是必须的（2）。

1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

原厂资料：

Specificity / Sensitivity

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at threonine 334.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2.

Background

In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.