Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb

• 产品编号：CST-2997P      品牌：Cell Signaling Technology       原厂货号：2997P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb能够检测内源性的Thr246位点磷酸化的PRAS40蛋白。该单克隆抗体是由合成的人源的针对PRAS40蛋白Thr246位点的磷酸化肽段免疫动物而生产的。许多生长因子和激素诱导PI3K激酶信号通路，从而激活下游效应蛋白，例如丝氨酸/苏氨酸激酶Akt（1,2）。一个已知的Akt的底物是一个40 kDa的，富含脯氨酸的蛋白（PRAS40），可以结合14-3-3蛋白（2）。PRAS40还能够结合mTOR，传递Akt信号到mTOR复合物。抑制mTOR信号可以刺激PRAS40结合到mTOR上，反过来抑制mTOR的活性（3）。在缺失胰岛素的细胞内PRAS40和mTOR复合物1（mTORC1）中的Raptor 结合，进而抑制细胞周期蛋白Rheb调节的mTORC1信号通路的激活。Akt磷酸化PRAS40的Thr246位点，解除PRAS40对mTORC1的抑制（4）。反过来mTORC1磷酸化PRAS40的Ser183（5）。

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
3. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
4. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
5. Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.

原厂资料：

Specificity / Sensitivity

Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr246 of human PRAS40.

Background

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).

1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
3. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
4. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
5. Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.