Rabbit (DA1E) mAb IgG XP? Isotype Control (Alexa Fluor? 647 Conjugate)

产品编号：CST-2985S 品牌：CST 原厂货号：2985S
产品分类：抗体 > 抗体制备 > 同型抗体对照Isotype controls
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包装：

运保温度:

4°C.

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描述：

Rabbit (DA1E) mAb IgG XP™同型对照不能直接用于其它已知抗原的检测，它的功能是作为兔单克隆抗体的同型对照。本抗体在最佳条件下采用Alexa Fluor ® 647荧光染料标记，已经过内部测试，可以直接用于人类细胞的流式细胞分析。同型对照是用来评估针对由于Fc受体结合或者其它蛋白与蛋白之间的相互作用引起的一抗的非特异性结合。一个同型对照抗体应该与被测抗体具有相同的免疫球蛋白类型和相同的浓度。

原厂资料：

Specificity / Sensitivity

Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) is not expected to recognize any specific antigen. It functions as an isotype control for rabbit monoclonal antibodies.

Source / Purification

The purified antibody was conjugated to Alexa Fluor® 647 under optimal conditions with a F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody was conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.