Lck (D88) XP? Rabbit mAb

• 产品编号：CST-2984S      品牌：CST       原厂货号：2984S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lck (D88) XP® Rabbit mAb 能够检测内源Lck蛋白总体水平。此抗体不与Src家族的其它成员交叉反应。此单克隆是通过合成人源对应的Lck Thr50位点周围的肽段来免疫动物而获得。Lck 属于Src样无受体酪氨酸激酶家族，具有典型的Src家族激酶的结构: 一个独特的N-末端域(Src同源4结构域, SH4) 然后是一个SH3 结构域, 一个SH2 结构域, 激酶结构域(SH1)和C-末端负调节结构域(1)。Lck的活性受到SH2和SH3区域的相互作用和在活性环内酪氨酸磷酸化的状态的共同调控(2,3)。受到刺激后，Lck蛋白被招募到T细胞受体(TCR)复合体上并激活下游酪氨酸激酶起始的T细胞信号通路(4)。Lck被发现也参与到线粒体凋亡信号通路的调控中，可能也对一些抗癌药物诱导凋亡作出反应(5,6)。

1. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
2. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15-27.
3. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112-21.
4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.
5. Belka, C. et al. (2003) Oncogene 22, 176-85.
6. Gruber, C. et al. (2004) Biochem Pharmacol 67, 1859-72.

原厂资料：

Specificity / Sensitivity

Lck (D88) XP® Rabbit mAb detects endogenous levels of total Lck protein. This antibody does not cross-react with other Src family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr50 of human Lck.

Background

Lck belongs to the Src-like non-receptor tyrosine kinase family with the typical Src family kinase structure: a unique amino terminal domain (Src homology 4 domain, SH4) followed by an SH3 domain, an SH2 domain, a kinase domain (SH1), and a carboxy-terminal negative regulatory domain (1). Lck activity is controlled by the interactions of SH2 and SH3 domains as well as tyrosine phosphorylation status of the activation loop (2,3). Lck is recruited to the T cell receptor (TCR) complex upon stimulation and activates downstream tyrosine kinases to initiate T cell signaling (4). Lck is also found to be involved in the regulation of mitochondrial apoptosis pathways and may be responsible for some anticancer drug induced apoptosis (5,6).

1. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
2. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15-27.
3. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112-21.
4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.
5. Belka, C. et al. (2003) Oncogene 22, 176-85.
6. Gruber, C. et al. (2004) Biochem Pharmacol 67, 1859-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.