Phospho-SRC-3 (Thr24) Antibody

• 产品编号：CST-2979S      品牌：CST       原厂货号：2979S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SRC-3 (Thr24) Antibody能够检测仅在Thr24位点磷酸化的内源性SRC-3蛋白水平。该抗体不与其它家族成员发生交叉反应。通过合成的与人源SRC-3蛋白Thr24位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。steroid receptor co-activator (SRC) 家族有三个成员蛋白：RC-1 (NCoA-1)、SRC-2 (TIF2/GRIP1/NCoA-2)和SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1)。所有SRC家族蛋白都有共同的结构同源性和功能，该功能是通过核激素受体和其他转录激活因子例如STAT3、NF-κB、E2F1和p53介导一个类似的方式去刺激转录（1-4）。两个SRC蛋白SRC-1和SRC-3都具有组蛋白乙酰转移酶的功能（5,6）。此外，所有的三个家族的蛋白都可以招募其他的组蛋白乙酰转移酶(CBP/p300, PCAF) 和组蛋白甲基转移酶(PRMT1, CARM1) 去定位到启动子并协助其增强许多基因的转录（5-8）。SRC蛋白在许多生理过程包括：细胞增殖，细胞存活，体细胞生长， 乳腺发育，雌性生殖功能以及血管保护中都起着重要作用（9）。SRC-1和SRC-3是激酶介导的生长因子信号传导到雌激素受体和其他转录激活因子的通道。7个SRC-1和6个SRC-3磷酸化位点已经被鉴定，这些位点的磷酸化由类固醇、细胞因子和生长因子所诱导，并且涉及到多个信号通路（9-11）。所有的3个SRC家族成员都与乳腺、前列腺和卵巢肿瘤核内受体的活性增加有关。此外，在多种癌症中SRC-3都频繁放大或过量表达（12），并且SRC-1/PAX3和SRC-2/MYST3的翻译分别与横纹肌肉瘤和急性髓细胞白血病有关联（13,14）。

SRC-3蛋白Thr24位点的磷酸化通过EGF刺激被诱导。磷酸化的SRC-3蛋白从细胞质定位到细胞核，在细胞核中该蛋白能与其它转录因子和类固醇激素受体相互作用，并且调节基因表达(15)。

1. Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011.
2. Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834.
3. Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171.
4. Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933.
5. Spencer, T.E. et al. (1997) Nature 389, 194-198.
6. Chen, H. et al. (1997) Cell 90, 569-580.
7. Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098.
8. Chen, D. et al. (1999) Science 284, 2174-2177.
9. Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
10. Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
11. Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
12. Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
13. Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545.
14. Deguchi, K. et al. (2003) Cancer Cell 3, 259-271.
15. Amazit, L. et al. (2007) Mol. Cell Biol. 27, 6913-6932.

原厂资料：

Specificity / Sensitivity

Phospho-SRC-3 (Thr24) Antibody detects endogenous levels of SRC-3 protein only when phosphorylated on Thr24. This antibody does not cross-react with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequence surrounding Thr24 of the human SRC-3 protein. Antibodies are purified by protein A and peptide affinity chromatography.Background

There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2) and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). The SRC family members all share significant structural homology and function in a similar fashion to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as STAT3, NF-κB, E2F1 and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines and growth factors and involve multiple kinase signaling pathways (9-11). All three SRC family members are associated with increased activity of nuclear receptors in breast, prostate and ovarian carcinomas. In addition, SRC-3 is frequently amplified or over-expressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).

Phosphorylation of Thr24 of SRC-3 proteins can be induced by stimulation with EGF. Phosphorylated SRC-3 translocates from the cytoplasm to the nucleus where it interacts with other transcription factors and steroid hormone receptors and regulates gene expression (15).

1. Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011.
2. Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834.
3. Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171.
4. Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933.
5. Spencer, T.E. et al. (1997) Nature 389, 194-198.
6. Chen, H. et al. (1997) Cell 90, 569-580.
7. Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098.
8. Chen, D. et al. (1999) Science 284, 2174-2177.
9. Wu, R.C. et al. (2004) Mol. Cell 15, 937-949.
10. Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483.
11. Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983.
12. Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274.
13. Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545.
14. Deguchi, K. et al. (2003) Cancer Cell 3, 259-271.
15. Amazit, L. et al. (2007) Mol. Cell Biol. 27, 6913-6932.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.