GFP (D5.1) XP? Rabbit mAb

• 产品编号：CST-2956P      品牌：Cell Signaling Technology       原厂货号：2956P
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

GFP (D5.1) XP ® Rabbit mAb用来检测外源性表达的具有GFP标签的蛋白。单克隆抗体是采用GFP氨基末端相对应的合成肽段免疫动物生产的。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。绿色荧光蛋白（GFP）在水母作为生物发光能量转移受体，其最大吸收光波长为395nm，发射谱的最大峰值为509nm。GFP作为一种融合蛋白已经成为报告基因表达，追踪细胞系和蛋白亚细胞定位的重要工具（1）。

Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

原厂资料：

Specificity / Sensitivity

GFP (D5.1) XP® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).

Miyawaki, A. et al. (2003) Curr Opin Chem Biol 7, 557-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.