FAAH1 (L14B8) Mouse mAb

• 产品编号：CST-2942S      品牌：CST       原厂货号：2942S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FAAH1 (L14B8)小鼠单克隆抗体识别内源性的FAAH1总蛋白水平。该单克隆抗体用与人类FAAH1蛋白氨基酸序列对应的人工合成肽段免疫动物制成。内源性大麻与成瘾行为和药物滥用相关（1）。脂肪酸酰胺水解酶1（FAAH1）是一个细胞膜结合的水解酶，将油酸酰胺转换为油酸（2）。此水解酶也能够转换内源性CB1大麻受体的配体大麻素成为花生四烯酸，表明其在脂肪酸酰胺灭活中有作用（2）。缺乏FAAH1的小鼠脑中有更高水平的花生四烯酸，显示出对疼痛的低敏感性，这进一步暗示了FAAH1在调节体内内源性大麻素信号通路中的作用（3）。FAAH1缺乏的小鼠与野生型小鼠相比，显示出对酒精偏爱的增加和自愿摄取酒精的增加，这表明FAAH1可能调控成瘾行为（1）。

1. Blednov, Y.A. et al. (2007) Neuropsychopharmacology 32, 1570-82.
2. Cravatt, B.F. et al. (1996) Nature 384, 83-7.
3. Cravatt, B.F. et al. (2001) Proc Natl Acad Sci USA 98, 9371-6.

原厂资料：

Specificity / Sensitivity

FAAH1 (L14B8) Mouse mAb detects endogenous levels of total FAAH1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human FAAH1.

Background

Endogenous cannabinoids have been implicated in addictive behaviors and drug abuse (1). Fatty-acid amide hydrolase 1 (FAAH1) is a plasma membrane-bound hydrolase that converts oleamide to oleic acid (2). This hydrolase also converts the cannabinoid anandamide, the endogenous ligand for the CB1 cannabinoid receptor, to arachidonic acid, suggesting a role in fatty-acid amide inactivation (2). Mice lacking FAAH1 have significantly higher levels of anandamide in the brain and show decreased sensitivity to pain, further indicating a role for FAAH1 in the regulation of endocannabinoid signaling in vivo (3). FAAH1 null mice also demonstrate an increased preference for alcohol and an increased voluntary uptake of alcohol as compared to wild-type mice, indicating a role of FAAH1 in modulating addictive behaviors (1).

1. Blednov, Y.A. et al. (2007) Neuropsychopharmacology 32, 1570-82.
2. Cravatt, B.F. et al. (1996) Nature 384, 83-7.
3. Cravatt, B.F. et al. (2001) Proc Natl Acad Sci USA 98, 9371-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.