Bim (C34C5) Rabbit mAb

• 产品编号：CST-2933S      品牌：CST       原厂货号：2933S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bim (C34C5) Rabbit mAb 兔单抗能够检测内源水平的Bim (EL, L 和S 形式) 总蛋白。单克隆抗体是采用合成 Bim 蛋白Pro25位点周围残基相对应的肽段来免疫动物而获得。Bim/Bod是促凋亡蛋白，属于Bcl-2家族只含有BH3域的成员，此家族还包括Bad、Bid、Bik、Hrk和 Noxa，它们都只包含BH3域，但缺少其它保守的BH1或BH2(1,2)。Bim通过结合并拮抗 Bcl-2家族抗凋亡因子而诱导凋亡。研究发现，其与Bcl-2、Bcl-xL、Mcl-1、Bcl-w、Bfl-1和 BHRF-1存在相互作用 (1,2)。Bim调控凋亡的功能是与胸腺细胞负向选择性有关，并伴随生长因子的撤回，在这期间Bim的表达是上调的(3-6)。Bim通过可变剪切，主要形成三种不同的形式：BimEL、BimL和BimS (1)。BimS是最短的形式，也是毒性最强的，它只在凋亡的瞬间表达。在凋亡过程中，BimEL 和 BimL 形式可能通过与动力蛋白的轻链互作，进而与动力蛋白复合体隔离并从这个复合体上得到释放(7)。这些长剪切形式的凋亡活性是通过磷酸化调控(8,9)。环境的胁迫引起JNK对Bim的磷酸化，并导致其与动力蛋白复合体的解离，从而增加凋亡活性。

1. O'Connor, L. et al. (1998) EMBO J 17, 384-95.
2. Hsu, S.Y. et al. (1998) Mol Endocrinol 12, 1432-40.
3. Bouillet, P. et al. (2002) Nature 415, 922-6.
4. Whitfield, J. et al. (2001) Neuron 29, 629-43.
5. Dijkers, P.F. et al. (2000) Curr Biol 10, 1201-4.
6. Ley, R. et al. (2003) J Biol Chem 278, 18811-6.
7. Puthalakath, H. et al. (1999) Mol Cell 3, 287-96.
8. Lei, K. and Davis, R.J. (2003) Proc Natl Acad Sci U S A 100, 2432-7.
9. Putcha, G.V. et al. (2003) Neuron 38, 899-914.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Dog, Pig

Specificity / Sensitivity

Bim (C34C5) Rabbit mAb detects endogenous levels of total Bim (EL, L and S isoforms) protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of Bim.

Background

Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2). Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.

1. O'Connor, L. et al. (1998) EMBO J 17, 384-95.
2. Hsu, S.Y. et al. (1998) Mol Endocrinol 12, 1432-40.
3. Bouillet, P. et al. (2002) Nature 415, 922-6.
4. Whitfield, J. et al. (2001) Neuron 29, 629-43.
5. Dijkers, P.F. et al. (2000) Curr Biol 10, 1201-4.
6. Ley, R. et al. (2003) J Biol Chem 278, 18811-6.
7. Puthalakath, H. et al. (1999) Mol Cell 3, 287-96.
8. Lei, K. and Davis, R.J. (2003) Proc Natl Acad Sci U S A 100, 2432-7.
9. Putcha, G.V. et al. (2003) Neuron 38, 899-914.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.