Phospho-PSD93 (Tyr340) Antibody

• 产品编号：CST-2930S      品牌：CST       原厂货号：2930S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PSD93 (Tyr340) Antibody 兔多抗识别内源性的Tyr340磷酸化的PSD93蛋白。该单克隆抗体由合成的人类PSD93蛋白Tyr340位点附近磷酸化肽段免疫动物而制成。突触后密集区蛋白93 (Postsynaptic Density protein 93,PSD93) 是一种膜相关鸟苷酸激酶（PSD - MAGUK）蛋白质PSD亚族的成员。结构上，它最类似于PSD95，由三个PDZ结构域后的可变N末端，一个SH3结构域和一个无效的鸟苷酸激酶（GK）结构域组成（1,2）。PSD93在神经细胞表达，定位于突出，与包括NMDA受体（2-4），K+通道（5-6），AMPA受体（7）等神经受体和蛋白质相互作用，调节自己的膜定位和神经信号。PSD93与持续性疼痛感应的突触后事件有关，是一种用于治疗此综合征的潜在的目标（3）。PSD93的Tyr340磷酸化位点由CST公司使用PhosphoScan®和CST的磷酸化位点质谱平台发现。PSD93在Tyr340上的磷酸化在缺血再灌注的大鼠脑组织分离提取物中可见。

1. Brenman, J.E. et al. (1996) J Neurosci 16, 7407-15.
2. Kim, E. et al. (1996) Neuron 17, 103-13.
3. Tao, Y.X. et al. (2003) J Neurosci 23, 6703-12.
4. Niethammer, M. et al. (1996) J Neurosci 16, 2157-63.
5. Kim, E. et al. (1995) Nature 378, 85-8.
6. Leyland, M.L. and Dart, C. (2004) J Biol Chem 279, 43427-36.
7. Elias, G.M. et al. (2006) Neuron 52, 307-20.

原厂资料：

Specificity / Sensitivity

Phospho-PSD93 (Tyr340) Antibody detects endogenous levels of PSD93 only when phosphorylated at Tyr340.

Source / Purification

Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr340 of human PSD93.

Background

Postsynaptic Density Protein 93 (PSD93) is a member of the PSD subfamily of the membrane-associated guanylate kinase (PSD-MAGUK) proteins. Structurally, it most closely resembles PSD95, consisting of an N-terminal variable segment followed by three PDZ domains, an SH3 domain and an inactive guanylate kinase (GK) domain (1,2) . PSD93 is expressed in neuronal cells and located at the synapse where it interacts with neuronal receptors and proteins including the NMDA receptor (2-4), K+ channels (5-6), and the AMPA receptor (7) to regulate their membrane localization and neuronal signaling. PSD93 is implicated in postsynaptic related persistent pain induction and is a potential target for treatment of this syndrome (3).The phosphorylation site at Tyr340 of PSD93 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PSD93 at Tyr340 was observed in extracts isolated from reperfusion of ischemic rat brain.

1. Brenman, J.E. et al. (1996) J Neurosci 16, 7407-15.
2. Kim, E. et al. (1996) Neuron 17, 103-13.
3. Tao, Y.X. et al. (2003) J Neurosci 23, 6703-12.
4. Niethammer, M. et al. (1996) J Neurosci 16, 2157-63.
5. Kim, E. et al. (1995) Nature 378, 85-8.
6. Leyland, M.L. and Dart, C. (2004) J Biol Chem 279, 43427-36.
7. Elias, G.M. et al. (2006) Neuron 52, 307-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.