Cyclin H Antibody

• 产品编号：CST-2927S      品牌：CST       原厂货号：2927S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cyclin H Antibody能够检测内源性cyclin H蛋白，且在生理水平不与家族内其他蛋白发生交叉反应。该多克隆抗体是由合成的针对cyclin H蛋白羧基末端肽段免疫动物，利用A蛋白和多肽亲和层析方法纯化生产的。细胞周期蛋白H属于一个保守的周期蛋白家族，在细胞周期循环必须的周期依赖性蛋白激酶(CDKs)调控个过程中发挥着重要作用(1,2)。一般情况下，CDKs的活性需要结合适当的周期蛋白以及CDK激活激酶(CAK)推动的磷酸化。周期蛋白H是CAK复合体的一部分，该复合体包括激酶CDK7和一个装配因子p36/Mat1，可以增强周期蛋白H和CDK7的结合并提高其活性(3,4)。CAK能够调节细胞周期的进程，通过CDK-周期蛋白复合体T回路一个关键苏氨酸残基的磷酸化激活cdc2, CDK2和CDK4激酶(5,6)。CAK复合体可以以游离形式才能在，也可以和能够影响其底物特异性的转录因子IIH (TFIIH)相结合(7,8,9)。与TFIIH结合以后，CAK优先磷酸化RNA聚合酶II的羧基末端结构域(9)，把细胞周期控制、转录调控和DNA修复联系在一起。

1. Fisher, R.P. and Morgan, D.O. (1994) Cell 78, 713-724.
2. Makela, T. P. et al. (1994) Nature 371, 254-257.
3. Yee, A. et al. (1995) Cancer Res. 55, 6058-6062.
4. Devault, A. et al. (1995) EMBO J. 14, 5027-5036.
5. Solomon, M.J. (1994) Trends Biochem. Sci. 19, 496-500.
6. Morgan, M.O. (1995) Nature 374, 131-133.
7. Shiekhatter, R. et al. (1995) Nature 374, 283-287.
8. Serizawa, H. et al. (1995) Nature 374, 280-282.
9. Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.

原厂资料：

Specificity / Sensitivity

Cyclin H Antibody detects endogenous levels of cyclin H. It does not cross-react with other family members at physiological levels.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of cyclin H. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cyclin H belongs to a conserved cyclin family that plays a critical role in the regulation of cell cycle dependent kinases (CDKs) necessary for cell cycle progression (1,2). In general, the activity of CDKs requires the binding of appropriate cyclins as well as phosphorylation driven by Cdk-activating kinase (CAK). Cyclin H is part of the CAK complex that includes the kinase CDK7, and an assembly factor p36/Mat1, which enhances binding between cyclin H and CDK7 and increases activity (3,4). CAK regulates progression through the cell cycle by activating cdc2, CDK2, and CDK4 kinases through phosphorylation of a critical threonine residue in the T-loop of the CDK-cyclin complexes (5,6). The CAK complex can exist either in its free form or in association with transcription factor IIH (TFIIH) which can affect its substrate specificity (7,8,9). When bound to TFIIH, CAK preferentially phosphorylates the carboxy-terminal domain of RNA polymerase II (9), providing a link between cell cycle control, transcriptional regulation, and DNA repair.

1. Fisher, R.P. and Morgan, D.O. (1994) Cell 78, 713-724.
2. Makela, T. P. et al. (1994) Nature 371, 254-257.
3. Yee, A. et al. (1995) Cancer Res. 55, 6058-6062.
4. Devault, A. et al. (1995) EMBO J. 14, 5027-5036.
5. Solomon, M.J. (1994) Trends Biochem. Sci. 19, 496-500.
6. Morgan, M.O. (1995) Nature 374, 131-133.
7. Shiekhatter, R. et al. (1995) Nature 374, 283-287.
8. Serizawa, H. et al. (1995) Nature 374, 280-282.
9. Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.