SOCS3 Antibody

• 产品编号：CST-2923S      品牌：CST       原厂货号：2923S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SOCS3抗体能够检测内源SOCS3蛋白总体水平。此多克隆抗体是通过合成对应的SOCS3 Lys195位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。SOCS (细胞因子信号抑制剂)家族成员能负调控细胞因子信号传导而抑制 Jak/Stat信号通路(1-3)。SOCS 家族中至少由8个成员组成，包括最初鉴定的CIS1蛋白 (细胞因子诱导的含有SH2域的蛋白)和从SOCS1到SOCS7 七个蛋白。每个SOCS 家族成员包含一个SH2中心域和C-末端域命名为SOCS盒。这些蛋白是细胞因子信号的重要调控者，包括细胞增殖，分化和免疫反应。SOCS3在肺，胸腺和脾脏中低水平表达，与 SOCS家族的其它成员一样，能够被很多的因素快速的激活，包括白介素, EPO, IFN-γ, CSF 和 TNF-α (4)。SOCS3 利用 SH2 区域结合激活的Jaks 及其同源的受体，然后提供负调控抑制。除了开始介绍的SOCS3的表达诱导因素, 随后的研究描述了SOCS3介导的负反馈抑制瘦素蛋白(5),GH (6),趋化因子受体(7),胰岛素(8)和一些特定的病原菌(9,10)。SOCS3敲除导致胚胎的胎盘功能不全所致的胚胎致死(11)。SOCS3信号通路与病原菌性过敏反应(12), 炎症疾病(13),内毒素休克(14),伤口愈合(15)和肥胖(16,17)有很大的关联性。

1. Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
2. Chen, X.P. et al. (2000) Immunity 13, 287-90.
3. Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
4. Starr, R. et al. (1997) Nature 387, 917-21.
5. Bjørbaek, C. et al. (1998) Mol Cell 1, 619-25.
6. Adams, T.E. et al. (1998) J Biol Chem 273, 1285-7.
7. Soriano, S.F. et al. (2002) J Exp Med 196, 311-21.
8. Emanuelli, B. et al. (2000) J Biol Chem 275, 15985-91.
9. Stoiber, D. et al. (1999) J Immunol 163, 2640-7.
10. Stoiber, D. et al. (2001) J Immunol 166, 466-72.
11. Roberts, A.W. et al. (2001) Proc Natl Acad Sci USA 98, 9324-9.
12. Seki, Y. et al. (2003) Nat Med 9, 1047-54.
13. Shouda, T. et al. (2001) J Clin Invest 108, 1781-8.
14. Fang, M. et al. (2005) Cell Mol Immunol 2, 373-7.
15. Goren, I. et al. (2006) J Invest Dermatol 126, 477-85.
16. Mori, H. et al. (2004) Nat Med 10, 739-43.
17. Howard, J.K. et al. (2004) Nat Med 10, 734-8.

原厂资料：

Specificity / Sensitivity

SOCS3 Antibody detects endogenous levels of total SOCS3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys195 of SOCS3. Antibodies were purified by protein A and peptide affinity chromatography.

Background

The SOCS (suppressor of cytokine signaling) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified protein CIS1 (cytokine-inducible SH2-containing protein) as well as SOCS1 through SOCS7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation and immune responses.Low levels of SOCS3 are observed in lung, spleen and thymus, and like other SOCS family members levels its expression is rapidly induced by a number of factors including interleukins, EPO, IFN-γ, CSF and TNF-α (4). SOCS3 uses its SH2 domain to bind activated Jaks and their cognate receptors to provide negative feedback inhibition. In addition to the initially described inducers of SOCS3 expression, subsequent studies have described SOCS3-mediated negative feedback inhibition for leptin (5), GH (6), chemokine receptors (7), insulin (8) and certain pathogens (9,10). SOCS3 deletion results in embryonic lethality with placental insufficiency (11). SOCS3 signaling has been linked pathologically to allergic responses (12), inflammatory disease (13), endotoxic shock (14), wound repair (15), and obesity (16,17).

1. Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
2. Chen, X.P. et al. (2000) Immunity 13, 287-90.
3. Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
4. Starr, R. et al. (1997) Nature 387, 917-21.
5. Bjørbaek, C. et al. (1998) Mol Cell 1, 619-25.
6. Adams, T.E. et al. (1998) J Biol Chem 273, 1285-7.
7. Soriano, S.F. et al. (2002) J Exp Med 196, 311-21.
8. Emanuelli, B. et al. (2000) J Biol Chem 275, 15985-91.
9. Stoiber, D. et al. (1999) J Immunol 163, 2640-7.
10. Stoiber, D. et al. (2001) J Immunol 166, 466-72.
11. Roberts, A.W. et al. (2001) Proc Natl Acad Sci USA 98, 9324-9.
12. Seki, Y. et al. (2003) Nat Med 9, 1047-54.
13. Shouda, T. et al. (2001) J Clin Invest 108, 1781-8.
14. Fang, M. et al. (2005) Cell Mol Immunol 2, 373-7.
15. Goren, I. et al. (2006) J Invest Dermatol 126, 477-85.
16. Mori, H. et al. (2004) Nat Med 10, 739-43.
17. Howard, J.K. et al. (2004) Nat Med 10, 734-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.