CDK7 (MO1) Mouse mAb

• 产品编号：CST-2916P      品牌：Cell Signaling Technology       原厂货号：2916P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDK7 (MO1) Mouse mAb能够检测内源性CDK7总蛋白，该抗体不与其他CDKs发生交叉反应。 该单克隆抗体是由重组人CDK7蛋白免疫动物生产的。CDK激活激酶(CAK)是CDK7和周期蛋白H的复合体。该复合体通过磷酸化cdks的T-回路结构域的激活残基参与细胞周期的调节(1)。CAK活性的调节经由T-回路的磷酸化和MAT1的缔合来介导，这两个过程都能够增强CAK对于RNA 聚合酶II(2,3)的CTD以及其它底物如p53(4)的激酶活性。CAK是转录复合体TFIIH必不可少的组成部分，并可以直接和TFIIH解旋酶相互作用(5)。

1. Fisher, R.P. et al. (1994) Cell 78, 713-724.
2. Larochelle, S. et al. (2001) EMBO J. 16, 3749-3759.
3. Yankulov, K.Y. et al. (1997) EMBO J. 16, 1638-1646.
4. Ko, L.J. et al. (1997) Mol. Cell. Biol. 17, 7220-7229.
5. Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.

原厂资料：

Specificity / Sensitivity

CDK7 (MO1) Mouse mAb detects endogenous levels of total CDK7 protein. The antibody does not cross-react with other CDKs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CDK7.

Background

CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5).

1. Fisher, R.P. et al. (1994) Cell 78, 713-724.
2. Larochelle, S. et al. (2001) EMBO J. 16, 3749-3759.
3. Yankulov, K.Y. et al. (1997) EMBO J. 16, 1638-1646.
4. Ko, L.J. et al. (1997) Mol. Cell. Biol. 17, 7220-7229.
5. Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.