Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb

• 产品编号：CST-2909S      品牌：CST       原厂货号：2909S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb兔单抗识别包含ATM/ATR底物基序的蛋白质。此抗体优先结合包含磷酸化丝氨酸/苏氨酸的肽段和蛋白质，还要求-1位有亮氨酸或相似的疏水氨基酸以及+1位是谷氨酰胺。此抗体不与相应的非磷酸化序列或其它包含磷酸化丝氨酸/苏氨酸的基序发生交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应专利）该单克隆抗体用合成的磷酸化ATM/ATR底物免疫动物制备。共济失调毛细血管扩张症突变蛋白激酶（ATM）和共济失调毛细血管扩张症Rad3相关激酶（ATR）是与细胞周期检查点和DNA修复相关的激酶（1）。已发现的ATM底物包括p53、p95/NBS1、MDM2、Chk2、BRCA1、CtIP、4E-BP1和Chk1（1,2）。ATM/ATR底物的核心要求是S*/T*Q。-3和-1位的疏水氨基酸和+1位的负电荷氨基酸对这些激酶底物识别有正向决定作用。S*/T*Q附近的正电荷残基对底物磷酸化有负向决定作用（3）。AT细胞的复杂表型提示它们还有更多底物（3）。为了更好的理解激酶和识别ATM和ATR的相关底物，CST开发了识别S*/T*Q模体中磷酸化丝氨酸和苏氨酸的抗体。

1. Kastan, M.B. and Lim, D.S. (2000) Nature Rev. Mol. Cell Biol. 1, 179-186.
2. Zhao, H. and Piwnica-Worms, H. (2001) Mol. Cell. Biol. 21, 4129-4139.
3. Kim, S. T. et al. (1999) J. Biol. Chem. 274, 37538-37543.

原厂资料：

Specificity / Sensitivity

Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb detects endogenous levels of proteins containing the ATM/ATR substrate motif. This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-ATM/ATR substrate peptides.

Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.

1. Kastan, M.B. and Lim, D.S. (2000) Nature Rev. Mol. Cell Biol. 1, 179-186.
2. Zhao, H. and Piwnica-Worms, H. (2001) Mol. Cell. Biol. 21, 4129-4139.
3. Kim, S. T. et al. (1999) J. Biol. Chem. 274, 37538-37543.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.