p70 S6 Kinase Substrates Antibody Sampler Kit

• 产品编号：CST-2903S      品牌：Cell Signaling Technology       原厂货号：2903S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
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描述：

p70 S6 Kinase Substrates Antibody Sampler Kit 里面的抗体都能够检测内源性的目标蛋白水平。 p70 S6 Kinase (49D7) Antibody #2708也识别 p85 S6 Kinase。Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb 兔单抗#9234 也识别Thr412位点磷酸化的 p85 S6 Kinase，还可能包括Thr401位点磷酸化的 S6KII。Kit里面的其他抗体不与其他蛋白交叉反应。抗体是由合成的针对人源的 S6 Kinase 氨基末端序列的肽段，及针对p70 S6 Kinase Thr389位点周围序列，核糖体蛋白Ser235和236位点 ，核糖体蛋白Ser240和244位点，eIF4B Ser422位点，eEF2k Ser366位点的磷酸化肽段免疫动物而生产的。多克隆抗体由protein A和肽亲和层析技术纯化。p70 S6 Kinase Substrates Antibody Sampler Kit为您提供一个经济快速的评定 p70 S6激酶若干底物的方法。Kit包含足够进行四次Western blot 实验所需的一抗和二抗。p70 S6激酶是有丝分裂原活化的丝氨酸/苏氨酸蛋白激酶，是细胞生长和G1期细胞周期正常进行必需的（1,2）。P70 S6激酶可以磷酸化核糖体40S亚基的S6蛋白并参与5‘ oligopyrimidine tract mRNAs的翻译控制（1）。核糖体蛋白S6的重要磷酸化位点存在于羧基末端的一小段含有Ser235, Ser236, Ser240, Ser244的区域（3，4）。 p70 S6激酶可以体内磷酸化修饰eIF4B的雷帕霉素敏感位点Ser422，体外翻译试验表明eIF4B Ser422位点突变为丙氨酸导致其活性显著降低（5）。 p70 S6 激酶和p90RSK磷酸化修饰eEF2K导致 eEF2K失活(6), 加速eEF2的去磷酸化最终促进翻译。

1. Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82.
2. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
3. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
4. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.
5. Raught, B. et al. (2004) EMBO J 23, 1761-9.
6. Wang, X. et al. (2001) EMBO J 20, 4370-9.

原厂资料：

Specificity / Sensitivity

Each antibody in the p70 S6 Kinase Substrates Antibody Sampler Kit detects endogenous levels of its target protein. p70 S6 Kinase (49D7) Antibody #2708 also recognizes p85 S6 Kinase. Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 also detects p85 S6 Kinase when phosphorylated at Thr412 and possibly S6KII when phosphorylated at Thr401. The other antibodies in the kit do not cross react with other proteins.

Source / Purification

Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 Kinase; and to synthetic phosphopeptides corresponding to residues surrounding Thr389 of human p70 S6 Kinase; Ser235 and 236 of human ribosomal protein; Ser240 and 244 of human ribosomal protein; Ser422 of human eIF4B; and Ser366 of human eEF2k. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The p70 S6 Kinase Substrates Antibody Sampler Kit provides a fast and economical means of evaluating several substrates of p70 S6 Kinase. The kit contains enough primary and secondary antibody to perform four Western blot experiments.

Background

p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs (1). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, Ser244) located wtihin a small, carboxy-terminal region of the S6 protein (3,4). p70 S6 kinase has been shown to phosphorylate eIF4B at the rapamycin-sensitive site Ser422 in vivo, and a Ser422Ala mutant of eIF4B shows diminished activity in an in vitro translation assay (5). Phosphorylation of eEF2K by p70 S6 kinase and p90RSK leads to inactivation of eEF2K (6), facilitating the dephosphorylation of eEF2 and thus promoting translation.

1. Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82.
2. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
3. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
4. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.
5. Raught, B. et al. (2004) EMBO J 23, 1761-9.
6. Wang, X. et al. (2001) EMBO J 20, 4370-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibodies.