CHOP (L63F7) Mouse mAb

• 产品编号：CST-2895S      品牌：CST       原厂货号：2895S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CHOP (L63F7) Mouse mAb识别内源性的CHOP总蛋白。该单克隆抗体用与人类CHOP蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。CHOP被鉴定为一种C/EBP同源蛋白，以显性负调控的方式抑制C/EBP和LAP（1）。CHOP表达可以被某些细胞应激所诱导，包括饥饿，诱导的CHOP抑制细胞周期从G1进入到S期（2）。后来发现，在内质网应激时，CHOP表达水平升高，可以介导程序性细胞死亡（3）。研究也发现CHOP可以介导GADD34和Ero1-Lα在内质网应激时的表达。GADD34反过来对eIF2α的磷酸化Ser51进行去磷酸化，从而刺激蛋白合成。Ero1-Lα促进内质网内氧化应激（4）。CHOP在内质网应激后细胞程序性死亡中的角色与其能够促进内质网内蛋白合成和氧化应激有关（4）。

1. Ron, D. and Habener, J.F. (1992) Genes Dev 6, 439-53.
2. Barone, M.V. et al. (1994) Genes Dev 8, 453-64.
3. Zinszner, H. et al. (1998) Genes Dev 12, 982-95.
4. Marciniak, S.J. et al. (2004) Genes Dev 18, 3066-77.

原厂资料：

Specificity / Sensitivity

CHOP (L63F7) Mouse mAb detects endogenous levels of total CHOP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CHOP.

Background

CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).

1. Ron, D. and Habener, J.F. (1992) Genes Dev 6, 439-53.
2. Barone, M.V. et al. (1994) Genes Dev 8, 453-64.
3. Zinszner, H. et al. (1998) Genes Dev 12, 982-95.
4. Marciniak, S.J. et al. (2004) Genes Dev 18, 3066-77.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.