Calreticulin Antibody

• 产品编号：CST-2891S      品牌：CST       原厂货号：2891S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Calreticulin（钙网蛋白）抗体识别内源性的全部calreticulin蛋白。该多克隆抗体用与人类钙网蛋白中Gln65附近氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白亲和层析纯化而得。钙是一种通用的信号传导分子，参与许多细胞功能，如细胞运动、代谢、蛋白质修饰、蛋白质折叠和细胞凋亡。钙存储于内质网（ER），在内质网内与钙离子结合分子伴侣如钙联接蛋白和钙网蛋白缓冲，并通过IP3受体通道释放（1）。钙网蛋白也作为内质网的分子伴侣起作用，以确保新合成糖蛋白的正确折叠和质量控制。因此，推测钙网蛋白不会改变蛋白质的折叠，而是在恰当的时间点调节有效折叠和亚基组装。此外，在内质网中，钙网蛋白以非天然构象保存，并对其进行靶向降解（2,3）。

1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

原厂资料：

Specificity / Sensitivity

Calreticulin Antibody detects endogenous levels of total calreticulin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Gln65 of human calreticulin. Antibodies are purified by peptide affinity chromatography.

Background

Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3).

1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.