TCF3/TCF7L1 (D15G11) Rabbit mAb

• 产品编号：CST-2883S      品牌：CST       原厂货号：2883S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TCF3 (D15G11)Rabbit mAb兔单抗识别内源性的总TCF3蛋白。此单抗由合成肽段免疫动物产生，该肽段与人TCF3 的邻近Gln96氨基酸残基序列一致.LEF1和TCF是高迁移率族蛋白(HMG)中DNA结合蛋白家族的成员中的一类转录因子，包括：Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1)。LEF1和TCF1最初被认为是调节早期淋巴发育的重要因子(2)，并作为Wnt的下游信号发挥作用。LEF1和TCF结合Wnt反应元件为β-catenin提供停泊位点，β-catenin随后转移到细胞核促进Wnt信号通路靶基因的转录激活(3)。在包括结肠癌（4，5）在内的多种癌症发生相关的Wnt信号通路的发展和异常活化中，都会伴随着LEF1和TCF表达水平的动态变化。TCF3（也称为TCF7L1），在参与干细胞分化进程的Wnt信号通路中起重要作用。

1. Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52.
2. Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32.
3. Brantjes, H. et al. (2002) Biol Chem 383, 255-61.
4. Reya, T. and Clevers, H. (2005) Nature 434, 843-50.
5. Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810.
6. Merrill, B.J. et al. (2001) Genes Dev. 15, 1688-1705.
7. Nguyen, H. et al. (2006) Cell 127, 171-183.
8. Cole, M.F. et al. (2008) Genes Dev. 22, 746-755.

原厂资料：

Specificity / Sensitivity

TCF3 (D15G11) Rabbit mAb detects endogenous levels of total TCF3 protein.Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln96 of human TCF3.Background

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).TCF3 (also known as TCF7L1) plays an important role in integrating Wnt signaling with control of stem cell differentiation (6-8).

1. Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52.
2. Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32.
3. Brantjes, H. et al. (2002) Biol Chem 383, 255-61.
4. Reya, T. and Clevers, H. (2005) Nature 434, 843-50.
5. Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810.
6. Merrill, B.J. et al. (2001) Genes Dev. 15, 1688-1705.
7. Nguyen, H. et al. (2006) Cell 127, 171-183.
8. Cole, M.F. et al. (2008) Genes Dev. 22, 746-755.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.